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ID 119545
著者
Hakoi, Haruka Tokushima University
Miki, Yoshimi Tokushima University|The University of Tokyo
Nomura, Saki Tokushima University
Nakajima, Kimiko Kochi University
Terashima-Murase, Chiaki Nagoya University
Takeichi, Takuya Nagoya University
Sano, Shigetoshi Kochi University
Akiyama, Masashi Nagoya University
Sakasegawa, Shin-ichi National Institute of Advanced Industrial Science and Technology|Asahi Kasei Pharma Corporation
Murakami, Makoto The University of Tokyo|Japan Agency for Medical Research and Development
山本, 圭 Tokushima University|Japan Agency for Medical Research and Development 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Group IIF phospholipase A2
Lysoplasmalogen
Thermocrispum lysophospholipase D
Psoriasis
Lipidomics
資料タイプ
学術雑誌論文
抄録
Epidermal lipids play important roles in skin homeostasis and diseases. Psoriasis is an inflammatory disease characterized by keratinocyte hyperproliferation and Th17 immune responses. We previously reported that ethanolamine-type lysoplasmalogen (P-LPE), preferentially produced by group IIF secreted PLA2 (sPLA2-IIF/PLA2G2F) that is expressed in the suprabasal epidermis, promotes epidermal hyperplasia in psoriatic inflammation. Herein, we show that forcible degradation of epidermal P-LPE by topical application of recombinant lysophospholipase D (LyPls-PLD) from Thermocrispum, a lysoplasmalogen-specific hydrolase, attenuated epidermal hyperplasia and inflammation in imiquimod-induced and K5.Stat3C-transgenic mouse psoriasis models. In humans, P-LPE levels were elevated in the tape-stripped stratum corneum of patients with psoriasis. Moreover, in primary cultured human epidermal keratinocytes, aberrant cell proliferation and activation by psoriatic cytokines were sPLA2-IIF/P-LPE-dependent and were suppressed by the addition of LyPls-PLD with a decrease in P-LPE. These findings confirm that the sPLA2-IIF/P-LPE axis in the epidermis indeed regulates psoriasis, that P-LPE is a lipid biomarker that predicts the severity of psoriasis, and that pharmacological removal of this bioactive lipid is useful to prevent the disease. Thus, our study may lead to the development of drug discovery and diagnostic techniques based on this pathway.
掲載誌名
Biochimie
ISSN
16386183
03009084
cat書誌ID
AA11522535
AA00564792
出版者
Elsevier|Société Française de Biochimie et Biologie Moléculaire (SFBBM)
215
開始ページ
75
終了ページ
87
発行日
2023-10-04
権利情報
© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
https://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
生物資源系