臨床検査分野へのマイクロチップ電気泳動の応用

Microfabricated devices are forecast to be fundamental to the postgenome era, especially in the field of genetics and medicine. Microchip electrophoresis has recently attracted much attention in the field of nuclear acid analysis due to the high efficiency, low consumption of sample and reagent, relatively low costs, and ease of operation. DNA fragments are separated by capillary electrophoresis in a microchip with microfabricated channels, with automated detection as well as on-line data evaluation. However, although there are many reports of the use of these instruments to evaluate standard DNA, DNA ladders, PCR products, and commercially available plasmid digests, little information is available their use with biological material. In this report, we showed the accuracy of sizing and quantification of endonuclease-digested pUC118. We also showed the feasibility of onchip endonuclease treatment of pUC118 and subsequent analysis as an additional application for DNA analysis. Furthermore, to evaluate the possibility of microchip electrophoresis for clinical laboratory, the results of the examination of blood glucose in human plasma and mitochondrial membrane potential were shown.

公開日:2010年1月24日で登録したコンテンツは、国立情報学研究所において電子化したものです。