ID 45160
タイトルヨミ
チョウコウネツキン コウソ オ ソシ ト スル バイオ センサー ノ カイハツ : ポリアミン カンレン コウソ ノ キノウ カイセキ ト D-プロリン ダツスイソ コウソ キノウ デンキョク センサー ノ カイハツ
タイトル別表記
Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Development of Biosensors Using Hyperthermophilic Enzymes as an Element:Functional Analysis of Polyamine Metabolizing Enzymes and Development for EnzymeElectrode Sensor Using D-Proline Dehydrogenases
著者
郷田, 秀一郎 徳島大学工学部生物工学科
櫻庭, 春彦 徳島大学工学部生物工学科
本仲, 純子 徳島大学工学部化学応用工学科 KAKEN研究者をさがす
大島, 敏久 徳島大学工学部生物工学科 徳島大学 教育研究者総覧
キーワード
enzyme sensor
hyperthermophile
dehydrogenase
polyamine
資料タイプ
紀要論文
抄録
An amperometric enzyme sensor give us higher sensitivity and specificity for the substrate
determination. In spite of advantages of enzyme sensor, many enzymes so far found have been too
labile to use as biosensor elements in artificial circumstances for a longer period.
Hyperthermophiles, which can grow above 90℃, have been known to produce much more stable
enzymes under various artificial conditions. In this work, we carried out screening, biochemical
characterization and improvement of production for hyperthermostable enzymes which are more useful
as the elements in the biosensors. We focused on the polyamines as one of the substrates of
biosensors. Polyamines have been known to play many important roles in cell proliferation,
differentiation and transformation. The concentration of the polyamines together with their acetyl
conjugates remarkably increases in the biological fluids and the affected tissues of cancer patients.
Therefore, their polyamines are listed as tumor markers. Gas and ion chromatographies have been
so far used for polyamine determination, but have some problems from the aspects of high sensitivity
and easy operation. Thus, we here developed biosensors using hyperthermostable enzymes for
polyamine determination. Such enzyme sensors are more useful for the simple and rapid
determination of polyamines and application for clinical analysis and food analysis In addition, we
tried the construction of biosensor using the hyperthermophilic enzyme, D-Proline dehydrogenase.
As the results, we found the thermostable agmatinase and spermidine dehydrogenase in
hyperthermophiles, Pyrococcus horikoshii and Sulfolobus tokodaii, respectively. We succeeded the
construction of novel amperometric sensor for D-proline determination using D-Proline dehydrogenase
derived from Pyrobaculum islandicum.
掲載誌名
徳島大学工学部研究報告
ISSN
03715949
cat書誌ID
AA1221470X
50
発行日
2005-03
EDB ID
121859
フルテキストファイル
言語
jpn
部局
理工学系
AWAサポートセンター
生物資源系