ID 79136
著者
河野, 崇 Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
田中, 克哉 Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Yin, hua Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
江口, 覚 Department of Dental Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
河野, 裕明 Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School
髙橋, 章 Department of Preventive Environment and Nutrition, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
中屋, 豊 Department of Nutrition and Metabolism, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
大下, 修造 Department of Anesthesiology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
intravenous anesthetics
ketamine
nicorandil
potassium channel
patch-clamp configuration
資料タイプ
学術雑誌論文
抄録
Purpose : Nicorandil opens adenosine triphosphate-sensitive potassium (KATP)
channels in the cardiovascular system and is being increasingly used for the treatment
of angina pectoris. In the present study, we tested whether intravenous anesthetic agent
ketamine affected nicorandil-induced native vascular KATP channel activation. Methods :
We used excised inside-out patch clamp configurations to investigate the direct effects
of ketamine racemate and S-(+)-ketamine on the activities of KATP channels in cultured
rat aortic smooth muscle cells. Furthermore, we also investigated whether intracellular
MgADP could modulate ketamine inhibition. Results : Nicorandil significantly activated
KATP channel activity, whereas this channel activity was completely blocked by glibenclamide,
a specific KATP channel blocker. Ketamine racemate inhibited the nicorandil induced
KATP channel activity (IC50=34 1M, n=14), but S-(+)-ketamine was less potent than ketamine
racemate in blocking nicorandil induced KATP channel activities (IC50=226 7M,
n=10). Application of MgADP to the intracellular side of the channel was able to decrease
the inhibitory potency of ketamine racemate on nicorandil induced KATP channel activities.
Conclusions : Our results indicate that ketamine inhibits nicorandil induced KATP channel
activities in a dose dependent and stereoselective manner. Furthermore, increase of
intracellularMgADP attenuates the inhibitory potency of ketamine racemate.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
57
3-4
開始ページ
237
終了ページ
244
並び順
237
発行日
2010-08
備考
The journal of medical investigation : http://medical.med.tokushima-u.ac.jp/jmi/index.html
EDB ID
フルテキストファイル
言語
eng
部局
医学系
歯学系
病院