ID 83812
著者
北川, 哲也 Department of Cardiovascular Surgery, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
マスダ, ユタカ Department of Cardiovascular Surgery, The University of Tokushima School of Medicine
富永, 崇司 Department of Cardiovascular Surgery, The University of Tokushima School of Medicine
カノウ, マサシ Department of Cardiovascular Surgery, The University of Tokushima School of Medicine
キーワード
cryopreservation
allograft valve
cellular viability
collagen synthesis
collagenolysis
matrix metalloproteinase
資料タイプ
学術雑誌論文
抄録
Although analyzing the precise mechanisms of cryopreserved allograft valve failure may be difficult due to a number of crucial reasons and the interrelationships between the overlapping mechanisms, there is some evidence that cryopreservation is currently the best method of storing allograft valves. The present review shows the basic cellular biology of cryopreserved allograft valves for long-term durability, particularly relevant to allograft valve cellular viability, the immune response mainly caused by viable donor cells, and the preservation and regeneration of the intrinsic extracellular matrix. The present findings are as follows. First, cryopreservation produces serious damage to cytosolic and mitochondrial functions of both endothelial cells and fibroblasts, which may cause valve failure after implantation. Second, although the collagen synthesis of cryopreserved valves was relatively maintained, total protein synthesis was highly diminished and the collagenolytic ability was activated immediately after the thawing process. These findings imply that the cryopreservation itself may cause the collagen metabolism to become degradable, which will lead to valve failure. Further examination of collagen metabolism and modulation of the collagenolytic activity will be necessary to improve the tissue preservation for improved clinical use.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
48
3-4
開始ページ
123
終了ページ
132
並び順
123
発行日
2001
備考
フルテキストファイル
言語
eng
部局
医学系