ID 74502
タイトル別表記
低酸素細胞特異的な蛍光イメージング剤の開発
著者
中田, 栄司 Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima
行待, 芳浩 Graduate School of Advanced Technology and Science, The University of Tokushima
阿部, 千秋 Graduate School of Advanced Technology and Science, The University of Tokushima
宇都, 義浩 Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
堀, 均 Division of Bioinformatics Engineering, Department of Life System, Institute of Technology and Science, Graduate School of the University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
Fluorescent pH probe
Tumor hypoxia
Fluorescent imaging
Bioreductive activation
Nitro reduction
資料タイプ
紀要論文
抄録
We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia
imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving
group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and
non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has
well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The
resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of
pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence
behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These
data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF
should monitor intracellular pH of tumor cells selectively with reduced background signal.
掲載誌名
徳島大学大学院ソシオテクノサイエンス研究部研究報告
ISSN
21859094
cat書誌ID
AA12214889
55
発行日
2010
フルテキストファイル
言語
eng
部局
生物資源系