ID 110716
著者
サカイ, マナブ Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
三宅, 秀則 Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
田代, 征記 Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
奥村, 裕司 Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima KAKEN研究者をさがす
木戸, 博 Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
FK 506
cyclosporine A
liver cancer cell
invasion
ICAM-1
資料タイプ
学術雑誌論文
抄録
Background : The prognosis of liver transplantation for liver cancer is determined by recurrence in the liver graft. In this study, the effects of immunosuppressors, FK506 and cyclosporine A (CsA) on the migration of liver cancer cells were investigated. Methods : The effects of FK506 at concentrations of 1-100 ng/mL and CsA at 1-1000ng/mL on the growth of poorly and well differentiated human hepatocellular carcinoma cell lines, HLE and HuH-7, respectively, were examined. After treatment of these cells with FK506 and CsA, the growth of these cells, their cytotoxicities and invasion assay on the Matrigel basement membrane invasion chamber were evaluated. In addition, the effects of FK506 and CsA on the changes in the production of a soluble intercellular adhesionmolecule-1 (sICAM-1) of these cells were measured. Results : FK506 and CsA at concentrations of 1-10 ng/mL inhibited the growth of both HLE and HuH-7 and those immunosuppressors at concentrations over 100 ng/mL exhibited cytotoxicity on these cells. FK506 at concentration of 1ng/mL significantly inhibited the invasion of poorly differentiated HLE, but not well differentiated HuH-7, after treatment for 2-5 days in culture (p<0.05), but FK 506 at 10 ng/mL showed less inhibitory efficient. CsA at concentrations of 1-10 ng/mL, however, did not inhibit or transiently inhibited the invasion of both cell lines. The production of ICAM-1 in HLE and HuH-7 was suppressed by FK506 at concentrations of 1-10 ng/mL after treatment for 3-5 days but the effect was not significant in the initial phase at days 1-2 and the last phase at days 5-6. Conclusions : FK506, but not CsA, at a clinical dose of 1ng/mL significantly inhibited the invasion of the poorly-differentiated HLE, but not HuH-7 and also inhibited the production of sICAM-1 in HLE.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
51
1-2
開始ページ
63
終了ページ
69
並び順
63
発行日
2004-02
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所
医学系