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ID 112153
タイトル別表記
インフルエンザ感染を制御する気道内酵素
Enzymes and inhibitors in airway that regulate infection of influenza virus
著者
村上, 明子 徳島大学
唐渡, 孝枝 徳島大学
大場, 久望子 徳島大学
キーワード
influenza virus
tryptase clara
mini-plasmin
pulmonary surfactant
mucus protease inhibitor (MPI)
資料タイプ
学術雑誌論文
抄録
It has been proposed that the pathogenicity of the influenza and Sendai virus is primarily determined by host cellular proteases that activate viral infectivity. We isolated trypsin-type serin proteases from rat lungs, candidates for the processing proteases of viral envelope glycoproteins, such as tryptase Clara localized in the Clara cells of the bronchial epithelium and mini-plasmin. These enzymes specifically cleave the precursor of fusion glycoprotein HA of influenza virus at Arg325, and the Fo of Sendai virus at Arg116 in the consensus cleavage motif, Gln(Glu)-X-Arg, resulting in the induction of infectivity of these viruses. Proteolytic activation of viruses by these enzymes occurs extracellularly, probably on the surface and/or in the lumen of the respiratory tract. On the other hand, we isolated two compounds from human bronchial lavage, which inhibitor the activity of tryptase Clara. One was a mucus protease inhibitor and the other was a pulmonary surfactant.
These compounds inhibited multiple cycles of virus replication in vitro and in vivo, but did not themselves affect the hemagglutination and the infectivity of the virus. Administration of these compounds in the airway may be useful for preventing and treating infection with influenza virus and Sendai virus.
掲載誌名
四国医学雑誌
ISSN
00373699
cat書誌ID
AN00102041
出版者
徳島医学会
55
5
開始ページ
174
終了ページ
179
並び順
174
発行日
1999-10-25
EDB ID
フルテキストファイル
言語
jpn
著者版フラグ
出版社版
部局
先端酵素学研究所