ID 113754
タイトル別表記
Bovine lactoferrin action on angiogenesis
著者
Tajima, Soichiro University of Tokushima
キーワード
lactoferrin
angiogenesis
eNOS
資料タイプ
学術雑誌論文
抄録
Lactoferrin (LF) exerts a variety of biological effects, including the promotion of angiogenesis by increasing the expression of angiogenesis-related genes and reducing blood pressure via a nitric oxide-dependent mechanism. In the present study, we investigated the effects of LF on angiogenesis using C57BL/6J mice that received daily unilateral treatment with or without bovine milk-derived LF (bLF) following unilateral hindlimb surgery. The analysis of laser speckle blood flow showed that bLF treatment promoted blood flow recovery in response to ischemic hindlimb. The capillary density of ischemic adductor muscles, as well as the phosphorylation of Src, Akt, and endothelial nitric oxide synthase (eNOS) was also significantly higher in bLF-treated mice than in vehicle-treated mice. Furthermore, bLF increased the phosphorylation levels of Src, Akt, and eNOS in in vitro experiments using human aortic endothelial cells (HAECs). The action of bLF on eNOS phosphorylation was abolished by both LY294002, a phosphatidylinositol 3-kinase inhibitor, and PP2, a Src inhibitor. Similarly, bLF-induced acceleration of tube formation, cell proliferation, and cell migration in HAECs were inhibited by LY294002 or PP2. Thus, bLF promotes vascular endothelial cell function via an Src-Akt-eNOS dependent pathway, thereby contributing to revascularization in response to ischemia.
掲載誌名
Journal of Cardiovascular Pharmacology
ISSN
15334023
01602446
cat書誌ID
AA00257104
出版者
Wolters Kluwer Health|Lippincott Williams & Wilkins
61
5
開始ページ
423
終了ページ
429
発行日
2013-05-01
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
医学系
薬学系