ID 110739
著者
池本, 哲也 Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
田代, 征記 Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
安友, 康二 Department of Immunology and Parasitology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キシハラ, ケンジ Department of Immunology and Parasitology, The University of Tokushima School of Medicine
栗田, 信浩 Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine KAKEN研究者をさがす
ミヤケ, ヒデノリ Department of Digestive and Pediatric Surgery, The University of Tokushima School of Medicine
キーワード
islet transplantation
experimental transplantation
donor-specific tolerance
CD4+CD25+ T-cells
mouse
資料タイプ
学術雑誌論文
抄録
Background. The allogeneic islets transplantation is an ideal therapeutic strategy for patients with diabetes mellitus. However, it has been difficult to induce immunological tolerance against islets grafts. TheCD4+CD25+ regulatory T-cells (Treg) play a role in suppressing T-cell activation. Thus, we evaluated whether Treg can regulate donor-specific T-cell tolerance that received allogeneic islets into the hepatic parenchyma (ITxHP) along with Treg. Methods. C3H/He mice were used as donors and streptozotocin-induced diabetic BALB/c mice were recipients. The protocol included three groups : Group A recipients received only 300 IE islets Group B was given 300 IE islets and whole splenocytes Group C was given 300 IE islets and Treg purified from peripheral lymph nodes. Results. For all mice in Groups A and B, the fasting blood sugar exceeded 250mg/dl and graft rejection was observed. GVHD was observed earlier in Group B than in Group A. In contrast graft survival exceeded 30 days for two mice in Group C (50%, mean POD 28.5±24.0, Plt0.05). Mixed lymphocyte reaction showed that T-cells from tolerant mice had very weak responses against spleen cells from C3H mice. Conclusions. The simultaneous ITxHP with CD4+CD25+ T-cells administration prolonged islet graft survivals and induced donor-specific hyporesposiveness.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
51
3-4
開始ページ
178
終了ページ
185
並び順
178
発行日
2004-08
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
病院
医学系