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ID 111119
タイトル別表記
DAPI染色による腎アミロイドーシスの新しい鑑別方法
Diagnosis of renal AL amyloidosis using DAPI
著者
Minakuchi, Jun Kawashima Hospital
キーワード
Amyloidosis
DAPI
Renal amyloidosis
AL amyloidosis
AA amyloidosis
資料タイプ
学位論文
抄録
Amyloidosis is often overlooked because its clinical manifestations can mimic those of more-common diseases. It is important to get a precise diagnosis as early as possible for the prevention of further organ damages. Amyloidosis is a disorder caused by deposition of insoluble abnormal amyloid. The kidney is a frequent site of amyloid deposition. The amyloid fibrils have a characteristic appearance and generate birefringence under polarized light when stained with the Congo red dye. Classification of amyloidosis is based on the precursor protein that forms the amyloid fibrils and the distribution of amyloid deposits as either systemic or localized. Involvement of amyloid fibrils in kidneys mainly occurs as amyloid light-chain (AL) or amyloid A (AA) amyloidosis. The potassium permanganate method with Congo red staining was once used widely to discriminate AL and AA amyloidoses, but thismethod has a problem of false positive results. We found that extracellular and cytoplasmic glomerular 4’,6-diamidino-2-phenylindole (DAPI)-positive areas were clearly consistent with amyloid deposition in AL amyloidosis. In contrast, the overlapping staining was not seen in AA amyloidosis. Therefore, we propose that DAPI staining readily distinguishes AL renal amyloidosis from AA renal amyloidosis as a simple and reproducible histochemical method.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
64
3-4
開始ページ
217
終了ページ
221
並び順
217
発行日
2017-08
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Motokazu Matsuuraの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2099号
学位記番号
乙医第1753号
学位授与年月日
2017-11-30
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系