ID | 114562 |
タイトル別表記 | JRAB shifts “dancing style” of cell clusters
|
著者 |
Yoshizawa, Shin
RIKEN
Nishimura, Masaomi
RIKEN
Tsuchiya, Yuko
National Institutes of Biomedical Innovation, Health and Nutrition|Osaka University
Matsushita, Natsuki
Ehime University|Aichi Medical University
Mizuguchi, Chiharu
Tokushima University
Ueno, Takato
Hokkaido University
Matsushita, Sachi
Ehime University
Haga, Hisashi
Hokkaido University
Deguchi, Shinji
Nagoya Institute of Technology|Osaka University
Mizuguchi, Kenji
National Institutes of Biomedical Innovation, Health and Nutrition
Yokota, Hideo
RIKEN
|
資料タイプ |
学術雑誌論文
|
抄録 | In fundamental biological processes, cells often move in groups, a process termed collective cell migration. Collectively migrating cells are much better organized than a random assemblage of individual cells. Many molecules have been identified as factors involved in collective cell migration, and no one molecule is adequate to explain the whole picture. Here we show that JRAB/MICAL-L2, an effector protein of Rab13 GTPase, provides the “law and order” allowing myriad cells to behave as a single unit just by changing its conformation. First, we generated a structural model of JRAB/MICAL-L2 by a combination of bioinformatic and biochemical analyses and showed how JRAB/MICAL-L2 interacts with Rab13 and how its conformational change occurs. We combined cell biology, live imaging, computational biology, and biomechanics to show that impairment of conformational plasticity in JRAB/MICAL-L2 causes excessive rigidity and loss of directionality, leading to imbalance in cell group behavior. This multidisciplinary approach supports the concept that the conformational plasticity of a single molecule provides “law and order” in collective cell migration.
|
掲載誌名 |
Molecular Biology of the Cell
|
ISSN | 19394586
|
出版者 | The American Society for Cell Biology
|
巻 | 27
|
号 | 20
|
開始ページ | 3095
|
終了ページ | 3108
|
発行日 | 2016-08-31
|
権利情報 | © 2016 Sakane et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|
部局 |
医学系
薬学系
|