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ID 109709
タイトル別表記
血管内皮細胞における酸化LDLおよびリゾホスファチジルコリン誘導によるカベオリン-1発現に対するケルセチンおよび代謝物の効果
著者
鎌田, 智英実 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Kondo, Akari Tokushima University
Sato, Shinya Tokushima University
キーワード
caveolin-1
adhesion molecule
quercetin
quercetin metabolite
vascular-endothelial cell
資料タイプ
学位論文
抄録
Oxidized low-density lipoprotein contributes to atherosclerotic plaque formation, and quercetin is expected to exert anti-atherosclerotic effects. We previously reported accumulation of conjugated quercetin metabolites in the aorta of rabbits fed high-cholesterol diets with quercetin glucosides, resulting in attenuation of lipid peroxidation and inhibition of lipid accumulation. Caveolin-1, a major structural protein of caveolae in vascular endothelial cells, plays a role in atherosclerosis development. Here we investigated effects of oxidized low-density lipoprotein, quercetin and its metabolite, quercetin 3-O-β-glucuronide, on caveolin-1 expression. Oxidized low-density lipoprotein significantly upregulated caveolin-1 mRNA expression. An oxidized low-density lipoprotein component, lysophosphatidylcholine, also induced expression of both caveolin-1 mRNA and protein. However, lysophosphatidylcholine did not affect the location of caveolin-1 proteins within caveolae structures. Co-treatment with quercetin or quercetin 3-O-β-glucuronide inhibited lysophosphatidylcholine-induced caveolin-1 expression. Quercetin and quercetin 3-O-β-glucuronide also suppressed expression of adhesion molecules induced by oxidized low-density lipoprotein and lysophosphatidylcholine. These results strongly suggest lysophosphatidylcholine derived from oxidized low-density lipoprotein contributes to atherosclerotic events by upregulating caveolin-1 expression, resulting in induction of adhesion molecules. Quercetin metabolites are likely to exert an anti-atherosclerotic effect by attenuating caveolin-1 expression in endothelial cells.
掲載誌名
Journal of Clinical Biochemistry and Nutrition
ISSN
18805086
09120009
cat書誌ID
AA10710201
AA12091064
出版者
Society for Free Radical Research Japan
58
3
開始ページ
193
終了ページ
201
発行日
2016-05-01
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201606151001.pdf
論文本文 : LID201608021001.pdf
著者の申請により要約(2016-06-15公開)に替えて論文本文を公開(2016-08-02)
本論文は, 著者Chiemi Kamadaの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
© 2016 Society for Free Radical Research Japan
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2945号
学位記番号
甲栄第233号
学位授与年月日
2016-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
生物資源系
医学系