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ID 113602
タイトル別表記
ANTI-TUMOR EFFICACY OF DOCETAXEL AND 5-FLUOROURACIL
著者
玉谷, 哲也 The University of Tokushima KAKEN研究者をさがす
Ferdous, Tarannum The University of Tokushima
Hara, Kanae The University of Tokushima
Kinouchi, Makoto The University of Tokushima
栗林, 伸行 The University of Tokushima KAKEN研究者をさがす
内田, 大亮 The University of Tokushima KAKEN研究者をさがす
永井, 宏和 The University of Tokushima KAKEN研究者をさがす
藤澤, 健司 The University of Tokushima KAKEN研究者をさがす
キーワード
oral squamous cell carcinoma
5-fluorouracil
docetaxel
sequential treatment
5-fluorouracil metabolic enzymes
資料タイプ
学術雑誌論文
抄録
Docetaxel (DOC) and 5-fluorouracil (5-FU) are important anticancer agents widely used in the treatment of a variety of cancers including oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the antitumor efficacy of the sequential administration of DOC and 5-FU against OSCC cells (B88 and CAL27 cells) in vitro and in vivo. In in vitro growth inhibition assays, sequential treatment with DOC followed by 5-FU was more effective in inhibiting cancer cell growth than 5-FU followed by DOC, single treatment with DOC or 5-FU, or combined treatment with DOC and 5-FU. Furthermore, DOC followed by 5-FU significantly inhibited tumor growth in vivo compared to 5-FU followed by DOC. To understand the mechanisms underlying the enhanced growth inhibitory effect of the administration sequence, DOC followed by 5-FU, we examined the expression of 5-FU metabolic enzymes such as thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD) and orotate phosphoribosyl transferase (OPRT), which were known to regulate the antitumor effect of 5-FU, by real-time RT-PCR and western blot analysis. Downregulation of TS and DPD expression and upregulation of OPRT expression were induced by DOC treatment, suggesting that DOC enhanced the efficacy of 5-FU by altering the expression of its metabolic enzymes. These results indicate that sequential treatment with DOC followed by 5-FU could be a promising therapeutic strategy for oral cancer.
掲載誌名
International Journal of Oncology
ISSN
10196439
17912423
cat書誌ID
AA10992511
出版者
Spandidos Publications
41
3
開始ページ
1148
終了ページ
1156
発行日
2012-07-04
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
病院
歯学系