ID | 109657 |
著者 |
石本, 恭子
Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School
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岩田, 武男
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
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谷口, 寿章
Division of Disease Proteomics, Institute for Enzyme Research, The University of Tokushima
徳島大学 教育研究者総覧
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水澤, 典子
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
徳島大学 教育研究者総覧
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田中, 栄二
Department of Orthodontics and Dentofacial Orthopedics, Institute of Health Biosciences, The University of Tokushima Graduate School
徳島大学 教育研究者総覧
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吉本, 勝彦
Department of Medical Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School
徳島大学 教育研究者総覧
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キーワード | D-dopachrome tautomerase
Preadipocyte
Adipokine
ERK
IL-6
Adipogenesis
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資料タイプ |
学術雑誌論文
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抄録 | We previously identified D-dopachrome tautomerase (DDT) as a novel adipokine whose mRNA levels in adipocytes are negatively correlated with obesity-related clinical parameters, and which acts on adipocytes to regulate lipid metabolism. Here we investigated functions of DDT on preadipocytes. Recombinant DDT (rDDT) enhanced both the expression and secretion of interleukin-6 (IL-6) in SGBS cells, a human preadipocyte cell line. Treatment with rDDT increased levels of phosphorylated ERK1/2, but not p38, in SGBS cells, and rDDT-induced IL-6 mRNA expression was attenuated by pretreatment with an ERK inhibitor, U0126. Knockdown of CD74, but not CD44, inhibited rDDT-induced IL-6 mRNA expression in SGBS cells. These results suggested that the rDDT-induced IL-6 expression in preadipocytes occurred through the CD74-ERK pathway. Furthermore, in SGBS cells subjected to adipogenic induction, rDDT decreased the amount of triacylglycerol, number of cells with oil droplets, and levels of mRNA encoding adipocyte marker proteins. Increased expression of CCAAT/enhancer binding protein families and peroxisome proliferation activated receptor γ2 during adipogenesis was inhibited in the cells treated with rDDT. These results suggested DDT to inhibit adipogenesis by suppressing the expression of genes encoding adipogenic regulators in preadipocytes.
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掲載誌名 |
Cytokine
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ISSN | 10434666
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cat書誌ID | AA10794201
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巻 | 60
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号 | 3
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開始ページ | 772
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終了ページ | 777
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並び順 | 772
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発行日 | 2012-12
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備考 | © 2012 Elsevier Ltd. All rights reserved.
© 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
著者版
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部局 |
歯学系
先端酵素学研究所
病院
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