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ID 114302
タイトル別表記
免疫欠損肺高血圧モデルマウスに対するヒト臍帯血由来単核球細胞移植の効果
Human cord blood-MNC transplantation improves PH
著者
Yoshida, Homare Tokushima University
Sugasawa, Noriko Tokushima University
キーワード
Pulmonary hypertension
cellular therapy
umbilical cord blood
monocrotaline
mononuclear cells
資料タイプ
学位論文
抄録
Objectives : To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods :MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results : Medial thickness, RV/S+ LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions : The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
AA12022913
出版者
Faculty of Medicine Tokushima University
64
1-2
開始ページ
43
終了ページ
49
並び順
43
発行日
2017-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mikio Suganoの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
乙第2109号
学位記番号
乙医第1757号
学位授与年月日
2019-12-26
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系