直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 116937
タイトル別表記
3D-clinorotationによるラットL6筋管萎縮に対するC14-Cblin及びcelastrolの同時投与による相加効果
Effect of Cblin and celastrol on muscle atrophy
著者
北畑, 香菜子 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Taniguchi, Runa Tokushima University
Kato, Ayano Tokushima University
Oarada, Motoko Sagami Women’s University
Junsoo, Park Yonsei University
Inho, Choi Yonsei University
キーワード
C14-Cblin
celastrol
Cbl-b
筋萎縮
酸化ストレス
myotube atrophy
muscle atrophy-associated ubiquitin ligases
oxidative stress
資料タイプ
学位論文
抄録
Two novel reagents, N-myristoylated Cbl-b inhibitory peptide (C14-Cblin) and celastrol, a quinone methide triterpene, are reported to be effective in preventing myotube atrophy. The combined effects of C14-Cblin and celastrol on rat L6 myotubes atrophy induced by 3D-clinorotation, a simulated microgravity model, was investigated in the present study. We first examined their effects on expression in atrogenes. Increase in MAFbx1/atrogin-1 and MuRF-1 by 3D-clinorotation was significantly suppressed by treatment with C14-Cblin or celastrol, but there was no additive effect of simultaneous treatment. However, celastrol significantly suppressed the upregulation of Cbl-b and HSP70 by 3D-clinorotation. Whereas 3D-clinorotation decreased the protein level of IRS-1 in L6 myotubes, C14-Cblin and celastrol inhibited the degradation of IRS-1. C14-Cblin and celastrol promoted the phosphorylation of FOXO3a even in microgravity condition. Simultaneous administration of C14-Cblin and celastrol had shown little additive effect in reversing the impairment of IGF-1 signaling by 3D-clinorotation. While 3D-clinorotation-induced marked oxidative stress in L6 myotubes, celastrol suppressed 3D-clinorotation-induced ROS production. Finally, the C14-Cblin and celastrol-treated groups were inhibited decrease in L6 myotube diameter and increased the protein content of slow-twitch MyHC cultured under 3D-clinorotation. The simultaneous treatment of C14-Cblin and celastrol additively prevented 3D-clinorotation-induced myotube atrophy than single treatment.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA11166929
出版者
Tokushima University Faculty of Medicine
69
1-2
開始ページ
127
終了ページ
134
並び順
127
発行日
2022-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kanako Kitahataの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3584号
学位記番号
甲栄第287号
学位授与年月日
2022-03-10
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系
病院