ID | 117434 |
著者 |
Senga, Yukako
National Institute of Advanced Industrial Science and Technology
Doi, Motomichi
National Institute of Advanced Industrial Science and Technology
Honda, Shinya
National Institute of Advanced Industrial Science and Technology
|
資料タイプ |
学術雑誌論文
|
抄録 | Recombinant immunoglobulin G (IgG) aggregates are formed during their production. However, the process underlying intracellular/extracellular aggregation in cell culture conditions is not well understood, and no effective method exists to assess IgG aggregates. Here, we establish an approach to detect intracellular aggregates using AF.2A1, a small artificial protein that binds to non-native IgG conformers and aggregates. Fluorescent-labeled AF.2A1 is prepared via conjugation and transfected into antibody-producing Chinese hamster ovary (CHO) cells. Micrographic images show intracellular IgG aggregates in CHO cells. The relative amount of intracellular aggregates (versus total intracellular IgG) differed depending on the type of additives used during cell culture. Interestingly, the relative amount of intracellular aggregates moderately correlates with that of in vitro extracellular IgG aggregates, suggesting they are secreted. This method will allow the investigation of antibody aggregation in cells, and may guide the production of therapeutic antibodies with high yield/quality.
|
掲載誌名 |
Cell Chemical Biology
|
ISSN | 24519456
|
出版者 | Elsevier
|
巻 | 29
|
号 | 1
|
開始ページ | 120
|
終了ページ | 132.e4
|
発行日 | 2021-11-04
|
権利情報 | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
出版社版
|
部局 |
生物資源系
|