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ID 117434
著者
Senga, Yukako National Institute of Advanced Industrial Science and Technology
Doi, Motomichi National Institute of Advanced Industrial Science and Technology
Honda, Shinya National Institute of Advanced Industrial Science and Technology
資料タイプ
学術雑誌論文
抄録
Recombinant immunoglobulin G (IgG) aggregates are formed during their production. However, the process underlying intracellular/extracellular aggregation in cell culture conditions is not well understood, and no effective method exists to assess IgG aggregates. Here, we establish an approach to detect intracellular aggregates using AF.2A1, a small artificial protein that binds to non-native IgG conformers and aggregates. Fluorescent-labeled AF.2A1 is prepared via conjugation and transfected into antibody-producing Chinese hamster ovary (CHO) cells. Micrographic images show intracellular IgG aggregates in CHO cells. The relative amount of intracellular aggregates (versus total intracellular IgG) differed depending on the type of additives used during cell culture. Interestingly, the relative amount of intracellular aggregates moderately correlates with that of in vitro extracellular IgG aggregates, suggesting they are secreted. This method will allow the investigation of antibody aggregation in cells, and may guide the production of therapeutic antibodies with high yield/quality.
掲載誌名
Cell Chemical Biology
ISSN
24519456
出版者
Elsevier
29
1
開始ページ
120
終了ページ
132.e4
発行日
2021-11-04
権利情報
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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言語
eng
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出版社版
部局
生物資源系