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ID 112386
タイトル別表記
歯髄象牙芽細胞の自然免疫反応におけるNOD1の役割
著者
細川, 由樹 徳島大学大学院口腔科学教育部(口腔科学専攻) 徳島大学 教育研究者総覧
Washio, Ayako Kyushu Dental University
Kitamura, Chiaki Kyushu Dental University
キーワード
Odontoblasts
KN-3
NOD1
pulpitis
資料タイプ
学位論文
抄録
Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
掲載誌名
BioMed Research International
ISSN
23146133
23146141
出版者
Hindawi
2016
開始ページ
9325436
発行日
2016
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261017.pdf
審査要旨:LID201704261018.pdf
論文本文:LID201704261019.pdf
本論文は,著者Yuki Hosokawaの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
Copyright ©2016 Yuki Hosokawa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3083号
学位記番号
甲口第426号
学位授与年月日
2017-03-23
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
病院