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ID 116380
タイトル別表記
ヒト角化上皮細胞における5-FU誘発性酸化ストレスおよび炎症応答に対するresveratrolの保護効果
著者
陳, 舒 徳島大学大学院口腔科学教育部(口腔科学専攻)
キーワード
oxidative stress
inflammation
oral mucositis
antioxidant
human normal keratinocyte
resveratrol
資料タイプ
学位論文
抄録
Although 5-fluorouracil (5-FU) is currently used as an anti-cancer chemotherapy, adverse effects such as oral mucositis potentially limit its clinical application. Additionally, the prevention of 5-FU-induced side effects are scarce. Resveratrol is known to decrease oxidative damage and inflammation. In this study, we examined the protective effects of resveratrol on 5-FU-induced oxidative stress and inflammatory responses in normal human keratinocytes (HaCaT cell) as in vitro oral mucositis model. HaCaT cells were exposed to 5-FU and simultaneously treated with resveratrol. The effects of resveratrol on 5-FU-induced cytotoxicity were evaluated using cell viability assay. The production of reactive oxygen species (ROS) was measured using a fluorescence spectrophotometer. The effects of resveratrol on nuclear factor erythroid 2-related factor 2 (Nrf2), silent information regulator transcript-1 (SIRT-1), and nuclear factor kappa B (NF-κB) signaling and inflammatory cytokine expression were examined. Resveratrol suppressed 5-FU-induced overproduction of ROS by upregulating anti-oxidant defense genes through Nrf2 activation and SIRT-1 expression. Concerning inflammatory responses, resveratrol suppressed the 5-FU-induced expression of pro-inflammatory cytokines via NF-κB nuclear translocation. Conversely, N-acetylcysteine reduced ROS levels without affecting the expression of pro-inflammatory cytokines. Resveratrol might be useful for preventing 5-FU-induced adverse effects by activating anti-oxidant and anti-inflammatory responses.
掲載誌名
Journal of Clinical Biochemistry and Nutrition
ISSN
09120009
18805086
cat書誌ID
AA10710201
出版者
Society for Free Radical Research Japan
69
3
開始ページ
238
終了ページ
246
発行日
2021-11-01
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Shu Chenの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3541号
学位記番号
甲口第472号
学位授与年月日
2021-09-09
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
医学系