ID 113324
タイトル別表記
子宮内膜症モデルマウスにおいてLPSは腹膜子宮内膜症初期病巣形成を促進する
LPS proliferate the endometriosis
著者
毛山, 薫 徳島大学大学院医科学教育部(医学専攻) KAKEN研究者をさがす
Kadota, Yuri Tokushima University
Erdenebayar, Otgontsetseg Tokushima University
Kasai, Kana Tokushima University
谷, 杏奈 Iseikai Hospital
松井, 寿美佳 Tokushima Red Cross Hospital KAKEN研究者をさがす
Maegawa, Masahiko Tokushima Prefectural Central Hospital
キーワード
LPS
endometriosis
inflammation
Murine model
cytokine
mice model
資料タイプ
学位論文
抄録
Purpose : The aims of this study were to clarify the effects of lipopolysaccharide (LPS) on the early development of endometriosis and on the production of cytokines and chemokines in the murine peritoneal cavity. Methods : Endometriotic lesions were induced in C57BL/6J adult female mice by intraperitoneal injection of endometrial fragments plus blood or endometrial fragments plus blood with LPS. On day 7, endometriotic lesions were assessed by gross and microscopic evaluations. Time-dependent changes in the secretion of TNF-α, IL-6, and CXCL2/MIP-2 in peritoneal lavage fluid after the intraperitoneal injection of LPS (50 µg/body) were measured by their respective enzyme-linked immunosorbent assays. Results : The areas of endometriotic lesions in the LPS group (10.8±8.6 mm2) were significantly larger than those in the control group (3.1±3.7 mm2). The levels of TNF-α and IL-6 peaked within 2 hours and the level of MIP-2 reached a maximum on day 1 after the injection of LPS. Conclusions : LPS promotes development of the early stages of murine endometriotic lesions.
掲載誌名
The Journal of Medical Investigation
ISSN
13496867
13431420
cat書誌ID
AA12022913
AA11166929
出版者
Tokushima University Faculty of Medicine
66
1-2
開始ページ
70
終了ページ
74
並び順
70
発行日
2019-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Kaoru Keyamaの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3248号
学位記番号
甲医第1397号
学位授与年月日
2018-12-27
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系