直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 116985
著者
Namula, Zhao Guangdong Ocean University|Tokushima University
Le, Quynh Anh Tokushima University
Lin, Qingyi Tokushima University
Takebayashi, Koki Tokushima University
Yoshimura, Naoaki Tokushima University
キーワード
CRISPR
electroporation
genome editing
porcine zygote
zona pellucida
資料タイプ
学術雑誌論文
抄録
Background: Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are unclear. In this study, the effects of ZP treatment before electroporation on embryonic development and gene editing in porcine embryos were evaluated.
Methods: The ZP of zygotes was weakened or removed by exposure to 0.5% actinase E, followed by electroporation of the Cas9 protein with guide RNA targeting GGTA1.
Results: The blastocyst formation rate of ZP-free zygotes after electroporation was significantly lower (p < 0.05) than that of ZP-intact zygotes. The mutation rate in blastocysts from ZP-weakened zygotes was similar to that in ZP-intact zygotes, whereas ZP removal increased the mutation rate. The mutation efficiency in blastocysts from electroporated zygotes did not differ among ZP treatment groups.
Conclusions: Our results indicate that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, whereas ZP removal has a detrimental effect on embryonic development but may increase the mutation rate.
掲載誌名
Veterinary Medicine and Science
ISSN
20531095
出版者
John Wiley & Sons
8
1
開始ページ
164
終了ページ
169
発行日
2021-10-21
権利情報
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系