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ID 116062
タイトル別表記
イソラムネチンはJ774.1マウスマクロファージ様細胞株においてTFEB非依存的にリソソームのタンパク質分解を促進する
Isorhamnetin activates lysosomes in J774.1 macrophages.
著者
坂井, 麻衣子 徳島大学大学院栄養生命科学教育部(人間栄養科学専攻)
Hara, Taichi Waseda University
キーワード
Isorhamnetin
TFEB
lysosome
macrophage
polyphenol
資料タイプ
学位論文
抄録
Lysosome is the principal organelle for the ultimate degradation of cellular macromolecules, which are delivered through endocytosis, phagocytosis and autophagy. The lysosomal functions have been found to be impaired by fatty foods and aging, and more importantly, the lysosomal dysfunction in macrophages has been reported as a risk of atherosclerosis development. In this study, we searched for dietary polyphenols which possess the activity for enhancing the lysosomal degradation in J774.1, a murine macrophage-like cell line. Screening test utilizing DQ-BSA digestion identified isorhamnetin (3’-O-methylquercetin) as an active compound. Interestingly, structural comparison to inactive flavonols revealed that the chemical structure of the B-ring moiety in isorhamnetin is the primary determinant of its lysosome-enhancing activity. Unexpectedly isorhamnetin failed to inhibit mTORC1-TFEB signaling, a master regulator of lysosomal biogenesis and function. Our data suggested that the other molecular mechanism might be critical for the regulation of lysosomes in macrophages.
掲載誌名
Bioscience, Biotechnology, and Biochemistry
ISSN
09168451
13476947
cat書誌ID
AA10824164
出版者
Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
84
6
開始ページ
1221
終了ページ
1231
発行日
2020-06-02
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Maiko Sakaiの学位論文として提出され,学位審査・授与の対象となっている
This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience, Biotechnology, and Biochemistry following peer review. The version of record Maiko Sakai, Kohta Ohnishi, Masashi Masuda, Hirokazu Ohminami, Hisami Yamanaka-Okumura, Taichi Hara, Yutaka Taketani, Isorhamnetin, a 3’-methoxylated flavonol, enhances the lysosomal proteolysis in J774.1 murine macrophages in a TFEB-independent manner., Bioscience, Biotechnology, and Biochemistry, Volume 84, Issue 6, 2 June 2020, Pages 1221–1231 is available online at: https://doi.org/10.1080/09168451.2020.1727309
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3509号
学位記番号
甲栄第283号
学位授与年月日
2021-03-23
学位名
博士(栄養学)
学位授与機関
徳島大学
部局
医学系