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ID 114776
著者
Tanikami, Yuki Tokushima University
Tagami, Takuma Tokushima University
Sakamoto, Mayu Tokushima University
キーワード
Acid dissociation constant
Flavin analogues
Instable substances
Capillary zone electrophoresis
資料タイプ
学術雑誌論文
抄録
Acid dissociation constants (pKa) of 9 kinds of flavin analogues as molecular catalyst candidates were determined by CZE. Although some of the analogues are instable and degradable under the light-exposure or in alkaline aqueous solutions, the effective electrophoretic mobility of the flavin analogue of interest has been measured with the residual substance. The pKa values of the flavin analogues were analyzed through the changes in the effective electrophoretic mobility with varying pH of the separation buffer. One or two steps pKa values were determined by the analysis. One of the degraded species from the flavin analogues, lumichrome, was also detected in the CZE analysis, and its pKa values were also determined. While coexisting impurities generated over the storage conditions were found in some analogues, the pKa values of the target analogues were successfully determined with the help of the CZE separations. A pressure-assisted CZE was utilized for the determination or the estimation of the pKa values of such analogues as possessing carboxylic acid moiety.
掲載誌名
Electrophoresis
ISSN
15222683
01730835
cat書誌ID
AA00179882
出版者
WILEY-VCH
41
15
開始ページ
1316
終了ページ
1325
発行日
2020-05-09
権利情報
This is the peer reviewed version of the following article: Tanikami, Y., Tagami, T., Sakamoto, M., Arakawa, Y., Mizuguchi, H., Imada, Y. and Takayanagi, T. (2020), Determination of acid dissociation constants of flavin analogues by capillary zone electrophoresis. ELECTROPHORESIS, 41: 1316-1325., which has been published in final form at https://doi.org/10.1002/elps.202000066. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
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フルテキストファイル
言語
eng
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著者版
部局
理工学系