直近一年間の累計
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ID 113044
著者
Obayashi, Mariko Hiroshima University
Yoshida, Maki Hiroshima University|Tokyo Medical University
Ogawa, Ikuko Hiroshima University
Sasahira, Tomonori Nara Medical University
Kuniyasu, Hiroki Nara Medical University
井本, 逸勢 The University of Tokushima KAKEN研究者をさがす
Abiko, Yoshimitsu Nihon University
Xu, Dan Hiroshima University|Dalian Maritime University
Fukunaga, Saori Hiroshima University
Tahara, Hidetoshi Hiroshima University
Nagao, Toshitaka Tokyo Medical University
高田, 隆 Hiroshima University
キーワード
invasion
microRNA (miRNA)
epithelial-mesenchymal transition (EMT)
head and neck squamous cell carcinoma (HNSCC)
microarray
資料タイプ
学術雑誌論文
抄録
Head and neck squamous cell carcinoma (HNSCC) has a high capacity for invasion. To identify microRNAs (miRNAs) that regulate HNSCC invasion, we compared miRNA expression profiles between a parent HNSCC cell line and a highly invasive clone. The miR-200 family and miR-203 were downregulated in the clone. Here we focused on the role of miR-203 in invasion and epithelial-mesenchymal transition (EMT) induction in HNSCC. miR-203 was downregulated during EMT induction. Moreover, ectopic overexpression of miR-203 suppressed the invasion and induced mesenchymal-epithelial transition (MET) in HNSCC cells. Interestingly, we identified NUAK family SNF1-like kinase 1 (NUAK1) as a novel target gene of miR-203 by cyclopedic analysis using anti-Ago2 antibody. Increased expression of NUAK1 was observed during EMT induction, and ectopic expression of miR-203 delayed EMT induction by suppressing NUAK1 expression. Moreover, NUAK1 overexpression promoted the invasion of HNSCC cells. Importantly, NUAK1 expression was well correlated with poor differentiation, invasiveness, and lymph node metastasis in HNSCC cases. Overall, miR-203 has a tumor-suppressing role in invasion and EMT induction by targeting NUAK1 in HNSCC, suggesting miR-203 as a potential new diagnostic and therapeutic target for the treatment of HNSCC.
掲載誌名
Oncotarget
ISSN
19492553
出版者
Impact Journals, LLC
7
7
開始ページ
8223
終了ページ
8239
発行日
2016-01-22
備考
This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0)(https://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系
歯学系