アクセス数 : ?
ダウンロード数 : ?
ID 113746
著者
Satoh, Akiho Tokushima University
Hamano, Hirofumi Tokushima University
Watanabe, Hiroaki Tokushima University
Imao, Mizuki Tokushima University
Hirayama, Tasuku Gifu Pharmaceutical University
永澤, 秀子 Gifu Pharmaceutical University
キーワード
muscle regeneration
muscle differentiation
p38MAPK
ERK1/2
iron
myogenesis
oxidative stress
mitogen-activated protein kinases(MAPKs)
資料タイプ
学術雑誌論文
抄録
Skeletal muscle atrophy is caused by disruption in the homeostatic balance of muscle degeneration and regeneration under various pathophysiological conditions. We have previously reported that iron accumulation induces skeletal muscle atrophy via a ubiquitin ligase-dependent pathway. However, the potential effect of iron accumulation on muscle regeneration remains unclear. To examine the effect of iron accumulation on myogenesis, we used a mouse model with cardiotoxin (CTX)-induced muscle regeneration in vivo and C2C12 mice myoblast cells in vitro. In mice with iron overload, the skeletal muscles exhibited increased oxidative stress and decreased expression of satellite cell markers. Following CTX-induced muscle injury, these mice also displayed delayed muscle regeneration with a decrease in the size of regenerating myofibers, reduced expression of myoblast differentiation markers, and decreased phosphorylation of mitogen-activated protein kinase signaling pathways. In vitro, iron overload also suppressed the differentiation of C2C12 myoblast cells, but the suppression could be reversed by superoxide scavenging using tempol. Excess iron inhibits myogenesis via oxidative stress, leading to an imbalance in skeletal muscle homeostasis.
掲載誌名
The FASEB Journal
ISSN
15306860
08926638
cat書誌ID
AA1066874X
出版者
Federation of American Societies for Experimental Biology|John Wiley & Sons
33
8
開始ページ
9551
終了ページ
9564
発行日
2019-05-30
備考
This is the pre-peer reviewed version of the following article: Ikeda, Y., Satoh, A., Horinouchi, Y., Hamano, H., Watanabe, H., Imao, M., Imanishi, M., Zamami, Y., Takechi, K., Izawa‐Ishizawa, Y., Miyamoto, L., Hirayama, T., Nagasawa, H., Ishizawa, K., Aihara, K.‐I., Tsuchiya, K. and Tamaki, T. (2019), Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress. Faseb, 33: 9551-9564, which has been published in final form at https://doi.org/10.1096/fj.201802724RR. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
医学系
病院
薬学系