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ID 118961
著者
Inoue, Yoshihiro Kyoto University
Phuong Vy, Trinh Thi Kyoto University
Singkaravanit-Ogawa, Suthitar Kyoto University
Zhang, Ru Kyoto University
Ogawa, Taiki Kyoto University
Ishizuka, Junya Kyoto University
Narusaka, Yoshihiro Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries
Takano, Yoshitaka Kyoto University
キーワード
Colletotrichum orbiculare
host specificity
phytopathogenic fungi
transcriptional control
virulence effector
資料タイプ
学術雑誌論文
抄録
The hemibiotrophic fungal plant pathogen Colletotrichum orbiculare is predicted to secrete hundreds of effector proteins when the pathogen infects cucurbit crops, such as cucumber and melon, and tobacco (Nicotiana benthamiana), a distantly related Solanaceae species. Here, we report the identification of sets of C. orbiculare effector genes that are differentially required for fungal virulence to two phylogenetically distant host species.
Through targeted gene knockout screening of C. orbiculare ‘core’ effector candidates defined based on in planta gene expression, we identified: four host-specific virulence effectors (named effector proteins for cucurbit infection, or EPCs) that are required for full virulence of C. orbiculare to cucurbit hosts, but not to the Solanaceae host N. benthamiana; and five host-nonspecific virulence effectors, which collectively contribute to fungal virulence to both hosts.
During host infection, only a small subset of genes, including the host-specific EPC effector genes, showed preferential expression on one of the hosts, while gene expression profiles of the majority of other genes, including the five host-nonspecific effector genes, were common to both hosts.
This work suggests that C. orbiculare adopts a host-specific effector deployment strategy, in addition to general host-blind virulence mechanisms, for adaptation to cucurbit hosts.
掲載誌名
New Phytologist
ISSN
0028646X
14698137
出版者
New Phytologist Foundation|John Wiley & Sons
238
4
開始ページ
1578
終了ページ
1592
発行日
2023-03-20
権利情報
This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系