ID | 118961 |
著者 |
Inoue, Yoshihiro
Kyoto University
Phuong Vy, Trinh Thi
Kyoto University
Singkaravanit-Ogawa, Suthitar
Kyoto University
Zhang, Ru
Kyoto University
Ogawa, Taiki
Kyoto University
Ishizuka, Junya
Kyoto University
Narusaka, Yoshihiro
Okayama Prefectural Technology Center for Agriculture, Forestry and Fisheries
Takano, Yoshitaka
Kyoto University
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キーワード | Colletotrichum orbiculare
host specificity
phytopathogenic fungi
transcriptional control
virulence effector
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資料タイプ |
学術雑誌論文
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抄録 | The hemibiotrophic fungal plant pathogen Colletotrichum orbiculare is predicted to secrete hundreds of effector proteins when the pathogen infects cucurbit crops, such as cucumber and melon, and tobacco (Nicotiana benthamiana), a distantly related Solanaceae species. Here, we report the identification of sets of C. orbiculare effector genes that are differentially required for fungal virulence to two phylogenetically distant host species.
Through targeted gene knockout screening of C. orbiculare ‘core’ effector candidates defined based on in planta gene expression, we identified: four host-specific virulence effectors (named effector proteins for cucurbit infection, or EPCs) that are required for full virulence of C. orbiculare to cucurbit hosts, but not to the Solanaceae host N. benthamiana; and five host-nonspecific virulence effectors, which collectively contribute to fungal virulence to both hosts. During host infection, only a small subset of genes, including the host-specific EPC effector genes, showed preferential expression on one of the hosts, while gene expression profiles of the majority of other genes, including the five host-nonspecific effector genes, were common to both hosts. This work suggests that C. orbiculare adopts a host-specific effector deployment strategy, in addition to general host-blind virulence mechanisms, for adaptation to cucurbit hosts. |
掲載誌名 |
New Phytologist
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ISSN | 0028646X
14698137
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出版者 | New Phytologist Foundation|John Wiley & Sons
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巻 | 238
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号 | 4
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開始ページ | 1578
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終了ページ | 1592
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発行日 | 2023-03-20
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権利情報 | This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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言語 |
eng
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著者版フラグ |
出版社版
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部局 |
生物資源系
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