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ID 115325
タイトル別表記
ミリプラチン及び放射線はPUMAを介したアポトーシスにより肝細胞癌に相乗効果を示す
Chemoradiotherapy with miriplatin
著者
田中, 宏典 徳島大学大学院医科学教育部(医学専攻)
Nakamura, Fumika Tokushima University
キーワード
hepatocellular carcinoma
miriplatin
radiation
synergistic effect
apoptosis
資料タイプ
学位論文
抄録
Background: The prognosis for patients with unresectable advanced hepatocellular carcinoma (HCC) is poor. Miriplatin is a hydrophobic platinum compound that has a long retention time in lesions after transarterial chemoembolization (TACE). We investigated anti-tumor activity of miriplatin combined with irradiation on HCC cells, and its underlying mechanism of apoptosis. We also analyzed the effectiveness of miriplatin-TACE and radiotherapy for locally advanced HCC.
Methods: Human HCC cell lines HepG2 and HuH-7 were treated with DPC (active form of miriplatin) and radiation, and synergy was evaluated using a combination index (CI). Apoptosis-related proteins and cell cycles were analyzed by western blotting and flowcytometry. We retrospectively analyzed treatment outcomes in 10 unresectable HCC patients with vascular/bile duct invasion treated with miriplatin-TACE and radiotherapy.
Results: DPC or X-ray irradiation decreased cell viability dose-dependently. DPC plus irradiation decreased cell viability synergistically in both cell lines (CI<1 respectively). Cleaved PARP expression was induced much more strongly by DPC plus irradiation than by each treatment alone. Expression of p53 up-regulated modulator of apoptosis (PUMA) was significantly induced by the combination, and knockdown of PUMA with siRNA significantly decreased apoptosis in both cell lines. DPC plus irradiation caused sub-G1, G2/M, and S phase cell arrest in those cells. The combination of miriplatin-TACE and radiotherapy showed a high response rate for patients with locally advanced HCC despite small number of patients.
Conclusions: Miriplatin plus irradiation had synergistic anti-tumor activity on HCC cells through PUMA-mediated apoptosis and cell cycle arrest. This combination may possibly be effective in treating locally advanced HCC.
掲載誌名
Journal of Gastroenterology
ISSN
09441174
14355922
cat書誌ID
AA10988015
AA11627089
出版者
Springer Nature|Japanese Society of Gastroenterology
55
11
開始ページ
1072
終了ページ
1086
発行日
2020-07-14
備考
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Hironori Tanakaの学位論文として提出され, 学位審査・授与の対象となっている。
This is a post-peer-review, pre-copyedit version of an article published in Journal of Gastroenterology.
The final authenticated version is available online at: https://doi.org/10.1007/s00535-020-01705-8
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3458号
学位記番号
甲医第1465号
学位授与年月日
2020-09-24
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院