ID 113977
タイトル別表記
CXCR3+ macrophage in Sjögren's syndrome
著者
Nakashiro, Koh-ichi Ehime University
キーワード
CXCR3
lip salivary gland
macrophage
Sjögren's syndrome
salivary gland
LSG
資料タイプ
学術雑誌論文
抄録
Background: Mechanisms underlying immune cells' recruitment and activation into the inflammatory lesions of lip salivary glands (LSGs) from primary Sjögren's syndrome (pSS) patients are incompletely understood. Chemokines play pivotal roles in these processes, so we investigated the clinical significance of chemokine receptor CXCR3 and its ligands in the autoimmune lesions of pSS.
Methods: We histologically determined the grade of LSG samples from 22 pSS patients and subjected the samples to immunofluorescence analysis to determine the expressions of CXCR3 and its ligands: CXCL9, CXCL10, and CXCL11. To identify the immune cells expressing CXCR3 in the LSGs, we performed double immunofluorescence analysis using antibodies against CD3 (pan-T cells), CD80 (M1 macrophages), CD163 (M2 macrophage), and CD123 (plasmacytoid dendritic cells: pDCs). The relationship between the grade of lymphocytic infiltration and the number of positively stained cells was analyzed by Spearman's rank correlation test.
Results: The expressions of CXCL9 and CXCL10 showed particularly intense staining in the LSG samples' ductal cells. The CXCR3 expression was detected mainly in CD80+ and CD163+ macrophages. The number of CXCR3+CD163+ macrophages inversely correlated with the LSG inflammatory lesions' severity (rs= −0.777, p<0.001).
Conclusions: Our results suggest that the enhanced production of CXCL9 and CXCL10 from ductal cells results in the CXCR3+ macrophages' migration. There was an inverse correlation between these two parameters: i.e., the number of CXCR3+CD163+ macrophages decreased as the lymphocytic infiltration grade increased. Although CXCR3 is expressed in all of the innate immune cells, CXCR3+CD163+ M2 macrophages may contribute to the anti-inflammatory functions in pSS lesions.
掲載誌名
Journal of Oral Pathology & Medicine
ISSN
09042512
16000714
cat書誌ID
AA10715555
出版者
John Wiley & Sons A/S
47
7
開始ページ
710
終了ページ
718
発行日
2018-06-21
備考
This is the peer reviewed version of the following article: Aota, K, Yamanoi, T, Kani, K, Nakashiro, K‐I, Ishimaru, N, Azuma, M. Inverse correlation between the number of CXCR3+ macrophages and the severity of inflammatory lesions in Sjögren's syndrome salivary glands: A pilot study. J Oral Pathol Med. 2018; 47: 710– 718, which has been published in final form at https://doi.org/10.1111/jop.12756. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
病院
歯学系