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ID 117221
著者
南川, 丈夫 Tokushima University|Japan Science and Technology Agency 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Morimoto, Yuki Tokushima University
Suzuki, Akihiro Tokushima University
Nakamura , Satoko Kagawa Prefectural Central Hospital
Tsutsui, Akemi Kagawa Prefectural Central Hospital
Takaguchi, Koichi Kagawa Prefectural Central Hospital
キーワード
second-harmonic generation microscopy
non-alcoholic fatty liver disease
non-alcoholic steatohepatitis
fibrosis
資料タイプ
学術雑誌論文
抄録
Non-alcoholic fatty liver disease (NAFLD) is associated with the chronic progression of fibrosis. In general, the progression of liver fibrosis is determined by a histopathological assessment with a collagen-stained section; however, the ultra-early stage of liver fibrosis is challenging to identify because of the low sensitivity in the collagen-selective staining method. In the present study, we demonstrate the feasibility of second-harmonic generation (SHG) microscopy in the histopathological diagnosis of the liver of NAFLD patients for the quantitative assessment of the ultra-early stage of fibrosis. We investigated four representative NAFLD patients with early stages of fibrosis. SHG microscopy visualised well-matured fibrotic structures and early fibrosis diffusely involving liver tissues, whereas early fibrosis is challenging to be identified by conventional histopathological methods. Furthermore, the SHG emission directionality analysis revealed the maturation of each collagen fibre of each patient. As a result, SHG microscopy is feasible for assessing liver fibrosis on NAFLD patients, including the ultra-early stage of liver fibrosis that is difficult to diagnose by the conventional histopathological method. The assessment method of the ultra-early fibrosis by using SHG microscopy may serve as a crucial means for pathological, clinical, and prognostic diagnosis of NAFLD patients.
掲載誌名
International Journal of Molecular Sciences
ISSN
14220067
出版者
MDPI
23
6
開始ページ
3357
発行日
2022-03-20
権利情報
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
理工学系
ポストLEDフォトニクス研究所
医学系