ID | 113980 |
著者 |
Hashimoto, Muneaki
National Institute of Advanced Industrial Science and Technology
Yatsushiro, Shouki
National Institute of Advanced Industrial Science and Technology
Yamamura, Shohei
National Institute of Advanced Industrial Science and Technology
Tanaka, Masato
National Institute of Advanced Industrial Science and Technology
Sakamoto, Hirokazu
National Institute of Advanced Industrial Science and Technology|The University of Tokyo
井戸, 佑介
National Institute of Advanced Industrial Science and Technology
Kajimoto, Kazuaki
National Institute of Advanced Industrial Science and Technology
片岡, 正俊
National Institute of Advanced Industrial Science and Technology
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キーワード | Malaria
Diagnosis
Automation
Hydrophilic treatment
Giemsa-staining
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資料タイプ |
学術雑誌論文
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抄録 | Background: Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable.
Results: A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia. Conclusion: Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria. |
掲載誌名 |
Malaria Journal
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ISSN | 14752875
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出版者 | BioMed Central|Springer Nature
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巻 | 16
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開始ページ | 321
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発行日 | 2017-08-08
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権利情報 | © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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言語 |
eng
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出版社版
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部局 |
先端酵素学研究所
病院
歯学系
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