直近一年間の累計
アクセス数 : ?
ダウンロード数 : ?
ID 118661
著者
Mizuno, Katsutoshi RIKEN
Shiozawa, Kei Osaka University|Otsuka Pharmaceutical
Katoh, Takanobu A. RIKEN
Minegishi, Katsura RIKEN|Osaka University
Ide, Takahiro RIKEN
Ikawa, Yayoi RIKEN|Osaka University
Nishimura, Hiromi RIKEN|Osaka University
Itabashi, Takeshi RIKEN
Iwane, Atsuko H. RIKEN
Nakai, Junichi Tohoku University
Shiratori, Hidetaka Osaka University|Kyoto Sangyo University
Hamada, Hiroshi RIKEN|Osaka University
資料タイプ
学術雑誌論文
抄録
Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.
掲載誌名
Science Advances
ISSN
23752548
出版者
American Association for the Advancement of Science
6
30
開始ページ
eaba1195
発行日
2020-07-22
権利情報
© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). (https://creativecommons.org/licenses/by-nc/4.0/)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
先端酵素学研究所