ID | 118661 |
著者 |
Mizuno, Katsutoshi
RIKEN
Shiozawa, Kei
Osaka University|Otsuka Pharmaceutical
Katoh, Takanobu A.
RIKEN
Minegishi, Katsura
RIKEN|Osaka University
Ide, Takahiro
RIKEN
Ikawa, Yayoi
RIKEN|Osaka University
Nishimura, Hiromi
RIKEN|Osaka University
Itabashi, Takeshi
RIKEN
Iwane, Atsuko H.
RIKEN
Nakai, Junichi
Tohoku University
Shiratori, Hidetaka
Osaka University|Kyoto Sangyo University
Hamada, Hiroshi
RIKEN|Osaka University
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資料タイプ |
学術雑誌論文
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抄録 | Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.
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掲載誌名 |
Science Advances
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ISSN | 23752548
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出版者 | American Association for the Advancement of Science
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巻 | 6
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号 | 30
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開始ページ | eaba1195
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発行日 | 2020-07-22
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権利情報 | © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). (https://creativecommons.org/licenses/by-nc/4.0/)
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言語 |
eng
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出版社版
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部局 |
先端酵素学研究所
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