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ID 115553
タイトル別表記
発育期分離すべりモデルラットにおける下位椎体変形修復の骨化様式
著者
眞鍋, 裕昭 徳島大学大学院医科学教育部(医学専攻)
石濱, 嘉紘 Tokushima University
キーワード
spondylolisthesis
rounding deformity
growth plate
endochondral ossification
transchondroid bone ossification
vertebral rounding deformity
mechanical stress
資料タイプ
学位論文
抄録
Study design: A study using rat spondylolisthesis models.
Objective: To elucidate the mechanism for correction of vertebral rounding deformity.
Summary of Background Data: Vertebral rounding deformity is the strongest risk factor for high-grade slippage associated with spondylolisthesis in adolescents. We previously reported that inadequate endochondral ossification of the anterior upper corner of the vertebral growth plate in response to mechanical stress could be the pathological mechanism of vertebral rounding deformity.
Methods: We created a model of spondylolisthesis using 4-week-old rats. They were divided into a tail suspension group that underwent tail suspension to decrease mechanical stress starting at 2 weeks postoperatively and a ground control group with no intervention. Radiographs and micro-computed tomography scans were obtained once weekly for 6 weeks postoperatively. The lumbar spines were then harvested for histological analysis. Immunohistochemical studies detected types I, II, and X collagen in the growth plate cartilage. Bone histomorphometrical analysis was also performed.
Results: Radiological and histological evidence in the ground control group showed progress the rounding deformity with time as previously reported. Formation of normal cancellous bone was observed radiologically over time in the tail suspension group, indicating correction of rounding deformity. Histologically, the site showing radiological evidence of correction was derived from cartilage tissue. After starting tail suspension, the growth plate stained positive for type X collagen and the corrected site stained for types II and X collagen in a mosaic pattern. Chondrocytes expressing types I and II collagen and tartrate-resistant acid phosphatase-positive cells were also present at the corrected site. Histomorphometrically, more endochondral bone was detected at the corrected site than in the posterior aspect of the normal growth plate.
Conclusion: Correction of vertebral rounding deformity was associated with improvement of chondrocyte differentiation, furthermore, there is possible involvement of a third mechanism, namely transchondroid bone ossification.
抄録別表記
It is possible to correct the rounding deformity of the vertebral body associated with isthmus slippage by decrease the load on the vertebral body in pediatric patients. In addition to improved chondrocyte differentiation and promotion of redifferentiation, there is possible involvement of a third mechanism, namely transchondroid bone ossification.
掲載誌名
Spine
ISSN
03622436
15281159
cat書誌ID
AA0084801X
AA12431865
出版者
Lippincott Williams & Wilkins|Wolters Kluwer Health, Inc.
発行日
2020-11-04
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Hiroaki Manabeの学位論文として提出され,学位審査・授与の対象となっている。
論文本文は2021-11-04以降に公開予定
権利情報
This the final peer-reviewed manuscript is made available under the CC-BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/)
出版社版DOI
出版社版URL
フルテキストファイル
言語
jpn
著者版フラグ
その他
文科省報告番号
甲第3468号
学位記番号
甲医第1473号
学位授与年月日
2020-12-24
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系