ID 112975
タイトル別表記
幼若雌ラットにおける発育に伴う卵巣Kiss1,Kiss1r遺伝子発現の変動およびHCGに対する感受性
OVARIAN Kiss1 mRNA IN THE PREPUBERTAL PERIOD
著者
山﨑, 幹雄 徳島大学大学院医科学教育部(医学専攻) KAKEN研究者をさがす
Taniguchi, Yuka Tokushima University
Yano, Yuya Tokushima University
キーワード
Development
kisspeptin
Kiss1
Kiss1r
Ovary
資料タイプ
学位論文
抄録
Kisspeptin, which is encoded by the Kiss1 gene, and its receptor, the G protein-coupled receptor 54 (Kiss1r), play important roles in the regulation of reproductive functions in mammals. Several studies have shown that the Kiss1 and Kiss1r genes are expressed in the rat, primate, and human ovaries, and that the ovarian kisspeptin system plays a pivotal role in ovulation at the proestrous stage in adulthood. The purpose of this study was to evaluate development-related changes in the expression of ovarian Kiss1 and Kiss1r genes and in kisspeptin levels, and to identify the regulatory factors for these genes during the prepubertal period. The serum kisspeptin level was also measured to examine whether ovarian kisspeptin affects serum kisspeptin levels. Variations in the ovarian Kiss1 and Kiss1r mRNA levels were observed during the prepubertal period in female rats, with levels peaking around postnatal days 20 and 15, respectively. Nevertheless, the ovarian kisspeptin content per total protein level was stably maintained. Serum kisspeptin levels at postnatal days 30 and 35 were higher than those at earlier postnatal days. The pattern of the ovarian Kiss1 mRNA levels was similar to that of the serum luteinizing hormone (LH) levels, and the ovarian Kiss1 mRNA level increased after injection with human chorionic gonadotropin (HCG) on postnatal day 20, but not on postnatal days 10 and 30. These data indicate that ovarian Kiss1 and Kiss1r mRNA levels are increased on postnatal days 20 and 15, respectively, and that changes in the serum LH level and the ovarian sensitivity to LH may be involved in the alteration of ovarian Kiss1 mRNA levels.
掲載誌名
Journal of Reproduction and Development
ISSN
13484400
09168818
cat書誌ID
AA10936678
出版者
Society for Reproduction and Development
63
4
開始ページ
409
終了ページ
414
発行日
2017-05-27
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mikio YAMASAKIの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
©2017 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3105号
学位記番号
甲医第1349号
学位授与年月日
2017-09-28
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系