ID 109780
著者
六車, 直樹 Department of Gastroenterology and Oncology, Institute of Health Biosciences, The University of Tokushima Graduate School|The Centre for Therapeutic Endoscopy and Endoscopic Oncology, St. Michael's Hospital 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
infrared fluorescence
probe
gastrointestinal cancer
資料タイプ
学術雑誌論文
抄録
The goal of our study is to develop a molecular imaging in gastrointestinal tract using infrared fluorescence endoscope (IRFE) and labeled antibodies with indocyanine green (ICG)-derivative in detecting cancerous tissue. IRFE consisted of an infrared endoscope equipped with excitation (710-790 nm) and barrier (810-920 nm) filters. We have developed ICG-N-hydroxy sulfo succinimide ester (ICG-sulfo-OSu) and 3-ICG-acyl-1,3-thiazolidine-2-thione (ICG-ATT) as an infrared fluorescent-labeling reagent. ICG-derivative-labeled anti-human carcinoembryonic antigen (CEA) antibody and MUC1 antibody were prepared. Freshly resected specimens with gastric cancer were observed by IRFE after reaction with ICG-derivative labeled antibodies. It resulted in positive fluorescence at the tumor location by IRFE, and the immnofluorescent images correlated well with the tumor sites. The immunohistochemical studies suggested the intensity of IR fluorescence of ICGATT-MUC1 stronger than ICG-sulfo-OSu. We conclude that an anti-CEA or MUC1 antibody with affinity for cancerous lesions and labeled with ICG-derivative can be imaged with this IRFE. Specific antibodies labeled with ICG-derivative with the reinforcing agent and electronic device can visualize cancer proteins generating a strong enough fluorescent signal.
掲載誌名
四国歯学会雑誌
ISSN
09146091
cat書誌ID
AN10050046
出版者
四国歯学会
21
2
開始ページ
333
終了ページ
339
並び順
333
発行日
2009-01-31
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系