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ID 30592
著者
カワハラ, ツカサ Department of Nutritional Physiology, The University of Tokushima School of Medicine
桑野, 由紀 Department of Nutritional Physiology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
近藤, 茂忠 Department of Nutritional Physiology, The University of Tokushima School of Medicine KAKEN研究者をさがす
河合, 智子 Department of Nutritional Physiology, The University of Tokushima School of Medicine
二川, 健 Department of Nutritional Physiology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
岸, 恭一 Department of Nutritional Physiology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
六反, 一仁 Department of Nutritional Physiology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
toll-like receptor
gastric pit cell
Helicobacter pylori
mitogen oxidase1
資料タイプ
学術雑誌論文
抄録
Gastric pit cells express mitogen oxidase1 (Mox1) and essential components for the phogocyte NADPH oxidase (p67-, p47-, p40-, and p22-phoxes). Helicobacter pylori (Hp) lipopolysaccharide (LPS) is a potent up-regulator of the Mox1 oxidase. In this study, we examined the expression levels of several key members of the Toll-like receptor (TLR) family in primary cultures of guinea pig gastric pit cells. These cells expressed the TLR4 mRNA. Immunoblot analysis and immunofluorescence histochemistry with an anti-TLR4 antibody showed that gastric pit cells possessed significant amounts of TLR4 protein preferentially on the plasma membrane. In contrast, the cells did not express the TLR2 and TLR9 transcripts and did not contain detectable amounts of TLR2 protein. Neither peptidoglycan from Staphylococcus aureus nor Hp DNA with the CpG motif up-regulated Mox1 oxidase activity. Hp LPS activated nuclear factor-κB in association with the expression of cyclooxygenase II and tumor necrosis factor α transcripts. These findings suggest that TLR4 may play a crucial role in the initiation of inflammatory responses of gastric pit cells against Hp infection.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
48
3-4
開始ページ
190
終了ページ
197
並び順
190
発行日
2001
備考
EDB ID
フルテキストファイル
言語
eng
部局
医学系