ID 106004
著者
ナカヤマ イマオウジ, ハルユキ Department of Immunology and Parasitology, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Molecular Bacteriology, Institute of Health Biosciences, the University of Tokushima Graduate School
イチムラ, ミノル Department of Immunology and Parasitology, Institute of Health Biosciences, the University of Tokushima Graduate School
イワサ, トモヤ Department of Molecular Bacteriology, Institute of Health Biosciences, the University of Tokushima Graduate School
オカダ, ナツミ Department of Molecular Bacteriology, Institute of Health Biosciences, the University of Tokushima Graduate School
大西, 克成 Department of Molecular Bacteriology, Institute of Health Biosciences, the University of Tokushima Graduate School 徳島大学 教育研究者総覧
桑原, 知巳 Department of Molecular Bacteriology, Institute of Health Biosciences, the University of Tokushima Graduate School|Department of Molecular Microbiology, Faculty of Medicine, Kagawa University
キーワード
Bacteroides fragilis
sialidase
sialoglycoconjugate
colonization
mucin
資料タイプ
学術雑誌論文
抄録
Recent analysis of the whole genome sequence of Bacteroides fragilis revealed extensive duplication of polysaccharide utilization genes in this anaerobe. Here we analyzed a unique 27-kb gene cluster (sgu) comprised of the 13 sialoglycoconjugates-utilization genes, which include the sialidase gene (nanH1) in B. fragilis strain YCH46. The genes were tightly organized and transcribed polycistronically. Comparative PCR scanning demonstrated that the sgu locus was conserved among the Bacteroides strains tested. Based on the transcriptional profiles generated by reverse transcriptase PCR, the sgu locus can be classified into at least three regulatory units : 1) sialic acid- or sialooligosaccharide-inducible genes, 2) constitutively expressed genes that can be down-regulated by catabolite repression, and 3) constitutively expressed genes. In vitro comparison of the growth of a sgu locus deletion mutant (SGUM172941) with a wild type strain indicates that this locus is necessary for B. fragilis to efficiently utilize mucin as a carbon source. Furthermore, SGUM172941 was defective in colonization of the intestines of germfree mice under competitive conditions. These data indicate that the sgu locus in B. fragilis plays a crucial role in the utilization of host-derived sialoglycoconjugates and the stable colonization of this anaerobe in the human gut.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
59
1-2
開始ページ
79
終了ページ
94
並び順
79
発行日
2012-02
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系