ID 110631
著者
Intiyot, Yaowarate Department of Bacteriology, The University of Tokushima School of Medicine|Department of Biochemistry, Faculty of Medicine, Chiang Mai University
キノウチ, タケミ Department of Bacteriology, The University of Tokushima School of Medicine
片岡, 佳子 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
有持, 秀喜 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
桑原, 知巳 Department of Bacteriology, The University of Tokushima School of Medicine
Vinitketkumnuen, Usanee Department of Biochemistry, Faculty of Medicine, Chiang Mai University
大西, 克成 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
キーワード
Murdannia loriformis
antimutagenicity
azoxymethane-induced aberrant crypt foci
O6-methylguanine
資料タイプ
学術雑誌論文
抄録
An 80% ethanol extract of Murdannia loriformis, a Thai medicinal plant, was examined for antimutagenic activity and cancer chemopreventive activity. In the Salmonella mutation assay, the extract showed antimutagenicity against 2-amino-3-methylimidazo [4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline, 2-amino-3,8-dimethylimidazo [4,5-f]quinoxaline, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 2-amino-1,4-dimethyl- 5H-pyrido[4,3-b]indole, 3-amino-1-methyl-5H-pyrido[4,3-b]indole, 2-amino-6-methyldipyrido [1,2-a:3’,2’-d] imidazole, 2-aminodipyrido[1,2-a:3’,2’-d]imidazole, 2-aminoanthracene, 2-(2- furyl)-3-(5-nitro-2-furyl) acrylamide,N-methyl-N’-nitro-N-nitrosoguanidineandmethylazoxymethanol acetate and reduced their mutagenicities to 31.4~67.9% at the dose of 10mg/plate. However, it did not inhibit the mutagenicities of 2-amino-9H-pyrido[2,3-b]indole, 2-amino-3-methyl-9 H-pyrido[2,3-b]indole, benzo[a]pyrene,N-ethyl-N’-nitro-N-nitrosoguanidine and 1-nitropyrene. The extract itself showed no mutagenicity. The chemopreventive activity of M. loriformis was examined using azoxymethane (AOM)-induced aberrant crypt focus (ACF) formation in the colon of F344 rats. The extract at doses of 0.1-1.0 g/kg wt significantly inhibited ACF formation in the initiation stage (21-51%), although it wasmore effective at a lower dose. In the post-initiation stage, the extract also tended to inhibit ACF formation (12 -27%) and significantly decreased the number of larger ACFs that have more than 3 aberrant crypts per focus. The extract inhibited the formation of O6-methylguanine and N7-methylguanine in the colonic mucosa and muscular layers but not or increased in the liver. These results indicate that M. loriformis extract has antimutagenic activity toward variousknownmutagens and that it inhibits AOM-induced ACF formation both in the initiation and post-initiation stages in the rat colon.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
49
1-2
開始ページ
25
終了ページ
34
並び順
25
発行日
2002
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
医学系