ID 29050
著者
桑原, 知巳 Department of Bacteriology, The University of Tokushima School of Medicine
中山, 治之 Department of Bacteriology, The University of Tokushima School of Medicine
ミキ, ツヨシ Department of Bacteriology, The University of Tokushima School of Medicine
片岡, 佳子 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
有持, 秀喜 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧 KAKEN研究者をさがす
大西, 克成 Department of Bacteriology, The University of Tokushima School of Medicine 徳島大学 教育研究者総覧
キーワード
Bacteroides fragilis
neuraminidase
oligonucleotide probe
septicemia
PCR
資料タイプ
学術雑誌論文
抄録
Bacteroides fragilis is a Gram-negative obligate anaerobe frequently isolated from clinical specimens and sometimes causes severe septicemia in compromised hosts. Increasing interest has been shown in the enterotoxigenicity and drug resistance of B. fragilis in the field of medical microbiology. We previously reported rapid detection of this anaerobe by nested PCR targeting a neuraminidase-encoding gene nanH. In the present study, we synthesized a digoxigenin-labeled oligonucleotide probe, NH1,which is specific for nanH of B. fragilis, and we combined the hybridization assay using NH1with the nanH-PCR to detect this anaerobe in a bacteremia model mice. In the specificity test, the oligonucleotide probe, NH1, hybridized only to amplification products from B. fragilis. PCR-dot blot hybridization based on nanH enabled detection of cells of B. fragilis in blood samples even when the number was as low as 2x103colony-forming units/ml. These findings suggest that PCR-dot blot hybridization targeting nanH is a useful procedure for diagnosis of septicemia caused by B. fragilis when viable cells in blood cannot be detected by the traditional culture techniques.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
48
1-2
開始ページ
60
終了ページ
65
並び順
60
発行日
2001
備考
EDB ID
フルテキストファイル
言語
eng
部局
医学系