ハギワラ, マリ Department of Molecular Bacteriology, Graduate School of Medicine, The University of Tokushima
片岡, 佳子 Department of Molecular Bacteriology, Graduate School of Medicine, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
有持, 秀喜 Department of Molecular Bacteriology, Graduate School of Medicine, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
桑原, 知巳 Department of Molecular Bacteriology, Graduate School of Medicine, The University of Tokushima
nonsteroidal anti-inflammatory drugs (NSAIDs)
Nonsteroidal anti-inflammatory drugs (NSAIDs) induced formation of intestinal ulcers as side effects, in which an unbalanced increase in the number of Gram-negative bacteria in the small intestine plays an important role. To clarify how intestinal microflora are influenced by NSAIDs, we examined the effects of 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (BFMeT), an NSAID, on intestinal motility and on the growth of Escherichia coli and Lactobacillus acidophilus. Transit index, a marker of peristalsis, was not different in BFMeT-treated and solvent-treated rats, indicating that BFMeT increased the number of Gram-negative bacteria without suppression of peristalsis. The factors that affect the growth of intestinal bacteria were not found in intestinal contents of BFMeT-treated rats, because the growth of E. coli and that of L. acidophilus in the supernatants of small intestinal contents of BFMeT-treated rats and solvent-treated rats were not different. The mechanism of the increase in the number of Gram-negative bacteria is still unclear, but heat-killed E. coli cells and their purified lipopolysaccharide (LPS) caused deterioration of BFMeT-induced ileal ulcers, while they could not cause the ulcers by themselves without the NSAID. Concentration of LPS and myeloperoxidase activity level were elevated correlatively in the intestinal mucosa of rats treated with LPS and BFMeT. These results suggest that an increase in the number of Gramnegative bacteria and their LPS in the mucosa induces activation of neutrophils together with the help of NSAID action and causes ulcer formation.
The journal of medical investigation : JMI
jmi_51_1-2_43.pdf 185 KB