アクセス数 : ?
ダウンロード数 : ?
ID 109646
著者
モリタニ, マキ Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima
吉本, 勝彦 Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
イイ, セツコ Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima
コンドウ, マキ Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima
イワハナ, ヒロユキ Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima
ヤマオカ, タカシ Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima
佐野, 壽昭 Department of Pathology, School of Medicine, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
ナカノ, ナオコ The Institute for Molecular and Cellular Biology, Osaka University
キクタニ, ヒトシ The Institute for Molecular and Cellular Biology, Osaka University
板倉, 光夫 Otsuka Department of Clinical and Molecular Nutrition, The University of Tokushima 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
insulin-dependent diabetes mellitus
insulitis
Th2
pancreatic islet B cells (islet B-cells)
somatic gene therapy
資料タイプ
学術雑誌論文
抄録
Four pancreatic islet-specific CD4+ helper T (Th) 1 (Th1) clones and two Th1 clones transduced with an SRα promoter-linked murine IL-10 (mIL-10) cDNA of 2.0–6.0×10[6] cells were adoptively transferred to nonobese diabetic (NOD) mice at age 8 d. Cyclophosphamide (CY) was administered at age 37 d (plus CY), and the incidence of diabetes and the histological grade of insulitis were examined at age 47 d. After the adoptive transfer of IL-10–transduced Th1 cells, polymerase chain reaction (PCR) and reversetranscription (RT)-PCR detected the neo gene and the retrovirus vector-mediated IL-10 mRNA in situ in recipient islets, respectively. RT-PCR detected the decrease of IFN-γ mRNA relative to IL-10 mRNA in IL-10–transduced Th1 clones in vitro and also in recipient islets. All four wild type Th1 clones plus CY induced the insulitis grade of 2.75 and diabetes in 66% of recipient NOD mice. IL-10–transduced two Th1 clones plus CY induced periinsulitis with the grade of 1.43 and diabetes in 8.0%. The 1:1 mixture of wild type Th1 cells and IL-10–transduced Th1 cells plus CY induced periinsulitis with the grade of 1.85 and diabetes in 20%. The suppression of diabetes through decreasing IFN-γ mRNA by the tissue-specific delivery of IL-10 to pancreatic islets with IL-10–transduced Th1 cells affords us the starting basis to develop the gene therapy for autoimmune diabetes.
掲載誌名
Journal of Clinical Investigation
ISSN
00219738
cat書誌ID
AA00695520
98
8
開始ページ
1851
終了ページ
1859
並び順
1851
発行日
1996-10-15
備考
© The American Society for Clinical Investigation, Inc.
EDB ID
出典
Journal of Clinical Investigation (1996) Vol.98 No.8 p.1851–1859
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
歯学系
医学系
先端酵素学研究所