ID 109529
タイトル別表記
歯原性上皮細胞におけるCtip2によるSp6遺伝子の転写制御
Role of Ctip2 in Sp6 regulation
著者
アディニンラト, アリヤ 徳島大学大学院口腔科学教育部(口腔科学専攻)
谷村, 綾子 the University of Tokushima KAKEN研究者をさがす
Yanuaryska, Ryna Dwi the University of Tokushima
ハギタ, ヒロコ the University of Tokushima
キーワード
ameloblast differentiation
Ctip2
Sp6
tooth development
transcriptional regulation
資料タイプ
学位論文
抄録
Tooth development relies on the interaction between the oral ectoderm and underlying mesenchyme, and is regulated by a complex genetic cascade. This transcriptional cascade is regulated by the spatiotemporal activation and deactivation of transcription factors.The specificity proteins 6 (Sp6) and chicken ovalbumin upstream promoter transcription factor-interacting protein 2 (Ctip2) were identified in loss-of-function studies as key transcription factors required for tooth development. Ctip2 binds to the Sp6 promoter in vivo ; however, its role in Sp6 expression remains unclear. In this study, we investigated Sp6 transcriptional regulation by Ctip2. Immunohistochemical analysis revealed that Sp6 and Ctip2 colocalize in the rat incisor during tooth development. We examined whether Ctip2 regulates Sp6 promoter activity in dental epithelial cells. Cotransfection experiments using serial Sp6 promoter-luciferase constructs and Ctip2 expression plasmids showed that Ctip2 significantly suppressed the Sp6 second promoter activity, although the Sp6 first promoter activity was unaffected. Ctip2 was able to bind to the proximal region of the Sp6 first promoter, as previously demonstrated, and also to the novel distal region of the first, and second promoter regions. Our findings indicate that Ctip2 regulates Sp6 gene expression through direct binding to the Sp6 second promoter region.
掲載誌名
The journal of medical investigation : JMI
ISSN
13431420
cat書誌ID
AA11166929
61
1-2
開始ページ
126
終了ページ
136
並び順
126
発行日
2014-02
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨 : LID201509181010.pdf
審査要旨 : LID201509181011.pdf
論文本文 : LID201509181012.pdf
本論文は,著者Arya Adiningratの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2859号
学位記番号
甲口第398号
学位授与年月日
2015-09-16
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系