ID | 112395 |
タイトル別表記 | 等張または低張条件下のラット耳下腺腺房細胞におけるα1-アドレナリン受容体誘導性のAQP5細胞内移動機序
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著者 |
ブラジエル, アネタ マグダレナ
徳島大学大学院口腔科学教育部(口腔科学専攻)
Wang, Di
Tokushima University|Fourth Military Medical University
Pieczonka, Tomasz D.
Tokushima University
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キーワード | aquaporin-5
α1A-adrenoceptor
α1B-adrenoceptor
α1D-adrenoceptor
calcium
protein G kinase
hypotonicity
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資料タイプ |
学位論文
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抄録 | Defective cellular trafficking of aquaporin-5 (AQP5) to the apical plasma membrane (APM) in salivary glands is associated with the loss of salivary fluid secretion. To examine mechanisms of α1-adrenoceptor (AR)-induced trafficking of AQP5, immunoconfocal microscopy and Western blot analysis were used to analyze AQP5 localization in parotid tissues stimulated with phenylephrine under different osmolality. Phenylephrine-induced trafficking of AQP5 to the APM and lateral plasma membrane (LPM) was mediated via the α1A-AR subtype, but not the α1B- and α1D-AR subtypes. Phenylephrine-induced trafficking of AQP5 was inhibited by ODQ and KT5823, inhibitors of nitric oxide (NO)-stimulated guanylcyclase (GC) and protein kinase (PK) G, respectively, indicating the involvement of the NO/ soluble (c) GC/PKG signaling pathway. Under isotonic conditions, phenylephrine-induced trafficking was inhibited by La3+, implying the participation of store-operated Ca2+ channel. Under hypotonic conditions, phenylephrine-induced trafficking of AQP5 to the APM was higher than that under isotonic conditions. Under non-stimulated conditions, hypotonicity-induced trafficking of AQP5 to the APM was inhibited by ruthenium red and La3+, suggesting the involvement of extracellular Ca2+ entry. Thus, α1A-AR activation induced the trafficking of AQP5 to the APM and LPM via the Ca2+/ cyclic guanosine monophosphate (cGMP)/PKG signaling pathway, which is associated with store-operated Ca2+ entry.
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掲載誌名 |
International Journal of Molecular Sciences
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ISSN | 14220067
16616596
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cat書誌ID | AA12038549
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出版者 | MDPI
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巻 | 17
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号 | 7
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開始ページ | 1022
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発行日 | 2016-06-28
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備考 | 内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704251013.pdf 審査要旨:LID201704251014.pdf 論文本文:LID201704251015.pdf 本論文は,著者Aneta M. Bragielの学位論文として提出され,学位審査・授与の対象となっている。 |
権利情報 | © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
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EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
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著者版フラグ |
博士論文全文を含む
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文科省報告番号 | 甲第3071号
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学位記番号 | 甲口第414号
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学位授与年月日 | 2017-03-23
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学位名 |
博士(学術)
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学位授与機関 |
徳島大学
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部局 |
技術支援部
歯学系
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