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ID 111987
著者
Ikeda, Mizuki Tokushima University
Deguchi, Junji Tokushima University
Fukushima, Shota Tokushima University
Qingyu, Ai Tokushima University
Katayama, Norihiko Tokushima University|Tohoku University
キーワード
Chlorothalonil
Intracellular Zn2+
Nonprotein thiol
Cytotoxicity
Lymphocyte
資料タイプ
学術雑誌論文
抄録
Chlorothalonil, a polychlorinated aromatic fungicide, is considered non-toxic to small mammals. However, chlorothalonil inactivates sulfhydryl enzymes and depletes cellular glutathione. Chlorothalonil increases intracellular Zn2+ concentration ([Zn2+]i) in mammalian cells possibly because intracellular Zn2+ is released via zinc-thiol/disulfide interchange. The effects of chlorothalonil at sublethal concentrations on the cellular content of nonprotein thiols ([NPT]i) and [Zn2+]i were examined using flow cytometry in rat thymocytes. Low concentrations (0.3–1 μM) of chlorothalonil increased, but high concentrations (3–10 μM) decreased [NPT]i. These effects of chlorothalonil were partly attenuated by an intracellular Zn2+ chelator. Chlorothalonil at 0.3–10 μM increased [Zn2+]i in a concentration-dependent manner, which was largely dependent on the release of intracellular Zn2+. Both the decrease in [NPT]i and increase in [Zn2+]i increase the vulnerability of cells to oxidative stress. Chlorothalonil at 1–10 μM potentiated the cytotoxicity of H2O2 (300 μM). It was also the case for 10 μM pentachloronitrobenzene, but not 10 μM pentachlorophenol. In conclusion, chlorothalonil at low (sublethal) micromolar concentrations is cytotoxic to mammalian cells under oxidative stress.
掲載誌名
Environmental Toxicology and Pharmacology
ISSN
13826689
cat書誌ID
AA1107880X
AA11526671
出版者
Elsevier
59
開始ページ
61
終了ページ
65
発行日
2018-03-08
権利情報
© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
著者版
部局
総合科学系
生物資源系