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ID 66467
著者
ハシモト, エリカ Faculty of Integrated Arts and Sciences, The University of Tokushima
キムラ, カオリ Faculty of Integrated Arts and Sciences, The University of Tokushima
カワナイ, タクヤ Faculty of Integrated Arts and Sciences, The University of Tokushima
ニシムラ, ユミコ Okayama University Dental School
小山, 保夫 Faculty of Integrated Arts and Sciences, The University of Tokushima KAKEN研究者をさがす
キーワード
zinc
nitroprusside
cytotoxicity
nitric oxide
資料タイプ
紀要論文
抄録
Nitric oxide (NO) is cytotoxic under some conditions although it has physiological roles. It is recently
proposed that the cytotoxicity of NO is resulted from its interaction with glutathione and zinc. Since we have
revealed that a decrease in cellular content of non-protein thiols, presumably glutathione, induces intracellular
Zn2+ release, there is a possibility that the cytotoxicity of nitroprusside, a donor of NO, is resulted from the
interaction of NO with cellular thiols, leading to an increase in intracellular Zn2+ concentration. To test the
possibility, the effects of nitroprusside on cell lethality, intracellular thiol content, and intracellular Zn2+
concentration were examined in rat thymocytes by using a flow cytometer with propidium iodide and FluoZin-3.
Nitroprusside at concentrations of 0.3 mM or more (up to 10 mM) significantly augmented FluoZin-3
fluorescence, indicating an increase in intracellular Zn2+ concentration. It was also the case under external
Zn2+-free condition, suggesting nitroprusside-induced release of intracellular Zn2+. However, nitroprusside at
10 mM did not affect cell lethality and cellular thiol content. Thus, it can be concluded that
nitroprusside-induced increase in intracellular Zn2+ concentration is not related to its cytotoxicity.
掲載誌名
徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
ISSN
09146385
cat書誌ID
AN10065859
出版者
徳島大学.総合科学部
24
2
開始ページ
7
終了ページ
12
並び順
7
発行日
2010-04
EDB ID
フルテキストファイル
言語
eng
著者版フラグ
出版社版
部局
生物資源系