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ID 111063
タイトル別表記
ロイテリン関連化合物の歯周病原因菌への抗菌および抗バイオフィルム効果
著者
藤原, 奈津美 徳島大学大学院口腔科学教育部(口腔科学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
Toguchi, Munehisa Tokushima University
キーワード
Lactobacillus reuteri
reuterin
Fusobacterium nucleatum
biofilm
資料タイプ
学位論文
抄録
OBJECTIVE: Lactobacillus reuteri is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by L. reuteri and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria.
MATERIALS AND METHODS: The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by Fusobacterium nucleatum were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes.
RESULTS: Among synthesized RRCs, RRC-04, -05, -09, -10, -12, -13, and -14, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-06, -07, -08, and -11, significantly suppressed biofilm formation to 60-80 % of the control. Most RRCs, except for RRC-12 and -13, were not cytotoxic to human oral keratinocytes.
CONCLUSIONS: The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.
掲載誌名
Journal of Oral Health and Biosciences
ISSN
21896682
出版者
四国歯学会
30
1
開始ページ
8
終了ページ
17
並び順
8
発行日
2017-06-30
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨:LID201704261002.pdf
審査要旨:LID201704261003.pdf
論文本文:k3076_fulltext.pdf
本論文は,著者Natsumi Fujiwaraの学位論文として提出され,学位審査・授与の対象となっている。
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3076号
学位記番号
甲口第419号
学位授与年月日
2017-03-09
学位名
博士(歯学)
学位授与機関
徳島大学
部局
歯学系
薬学系
病院