ID | 117824 |
タイトル別表記 | Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide
|
著者 |
Hasi, Rumana Yesmin
Tokushima University
Ishikawa, Toshiki
Saitama University
Sunagawa, Keigo
Tokushima University
Takai, Yoshimichi
Tokushima University
Ali, Hanif
Tokushima University
Imai, Hiroyuki
Konan University
|
キーワード | Glycosylinositol phosphoceramide
phospholipase D
phytoceramide 1-phosphate
nonspecific phospholipase C3
Raphanus sativus
|
資料タイプ |
学術雑誌論文
|
抄録 | Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of D position of glycosylinositol phosphoceramide (GIPC) in cabbage and called its activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as PLD. The recombinant NPC3 protein obtained by heterologous expression system with E. coli produced PC1P from GIPC, and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish, and A. thaliana. From these results, we concluded that NPC3 is one of the enzymes that degrade GIPC, a major sphingolipid in plants.
|
掲載誌名 |
FEBS Letters
|
ISSN | 18733468
|
cat書誌ID | AA00642943
|
出版者 | Federation of European Biochemical Societies|Wiley
|
巻 | 596
|
号 | 23
|
開始ページ | 3024
|
終了ページ | 3036
|
発行日 | 2022-10-20
|
権利情報 | This is the peer reviewed version of the following article: Hasi, R.Y., Ishikawa, T., Sunagawa, K., Takai, Y., Ali, H., Hayashi, J., Kawakami, R., Yuasa, K., Aihara, M., Kanemaru, K., Imai, H. and Tanaka, T. (2022), Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide. FEBS Lett. 596: 3024-3036. which has been published in final form at https://doi.org/10.1002/1873-3468.14520. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
|
EDB ID | |
出版社版DOI | |
出版社版URL | |
フルテキストファイル | |
言語 |
eng
|
著者版フラグ |
著者版
|
部局 |
生物資源系
薬学系
|