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ID 113692
タイトル別表記
肺腺癌患者の予後予測におけるGAD1過剰発現の有用性
著者
坪井, 光弘 徳島大学大学院医科学教育部(医学専攻) 徳島大学 教育研究者総覧 KAKEN研究者をさがす
増田, 清士 Tokushima University|Kawasaki Medical School KAKEN研究者をさがす
Kohmoto, Tomohiro Tokushima University
井本, 逸勢 Tokushima University|Aichi Cancer Center Research Institute|Nagoya University 徳島大学 教育研究者総覧 KAKEN研究者をさがす
キーワード
GAD1
lung adenocarcinoma
expression
prognosis
DNA methylation
資料タイプ
学位論文
抄録
Background and Objectives: In a previous genome-wide screening, we identified hypermethylated CpG islands around glutamate decarboxylase 1 (GAD1) in lung adenocarcinoma (LADC). In this study, we aimed to investigate the methylation and expression status of GAD1 and its prognostic value in patients with LADC.
Methods: GAD1 methylation and mRNA expression status were analyzed using 33 tumorous and paired non-tumorous LADC samples and publicly available datasets. The prognostic value of GAD1 overexpression was investigated using publicly available datasets of mRNA levels and 162 cases of LADC by immunohistochemistry.
Results: The methylation and mRNA expression levels of GAD1, each having a positive correlation, were significantly higher in LADC tumors than in paired non-tumorous tissues. LADC patients with higher GAD1 mRNA expression showed significantly poorer prognosis for overall survival in publicly available datasets. Higher immunoreactivity of GAD1 was significantly associated with the pathological stage, pleural invasion, lymph vessel invasion, and poorer prognosis for cancer-specific and disease-free survival. Multivariate analysis revealed that GAD1 protein overexpression is an independent prognosticator for disease-free survival.
Conclusions: GAD1 mRNA and protein expression levels were significant prognostic factors in LADC, suggesting that they might be useful biomarkers to stratify patients with worse clinical outcome after resection.
掲載誌名
Cancer Medicine
ISSN
20457634
出版者
John Wiley & Sons, Ltd
8
9
開始ページ
4189
終了ページ
4199
発行日
2019-06-17
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Mitsuhiro Tsuboiの学位論文として提出され,学位審査・授与の対象となっている。
権利情報
© 2019 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3321号
学位記番号
甲医第1423号
学位授与年月日
2019-07-25
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系