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ID 106314
タイトル別表記
成長軟骨板において膜フィルターで血管侵入を阻害すると肥大軟骨細胞は増殖し骨芽細胞様細胞に分化する。
Hypertrophic Chondrocytes Differentiate into Osteogenic Cells
著者
江西, 哲也 徳島大学大学院医科学教育部(医学専攻) KAKEN研究者をさがす
キーワード
軟骨細胞
成長軟骨版
軟骨内骨化
骨芽細胞
分化
資料タイプ
学位論文
抄録
The fate of hypertrophic chondrocytes during endochondral ossification remains controversial. It has long been thought that the calcified cartilage is invaded by blood vessels and that new bone is deposited on the surface of the eroded cartilage by newly arrived cells. The present study was designed to determine whether hypertrophic chondrocytes were destined to die or could survive to participate in new bone formation. In a rabbit experiment, a membrane filter with a pore size of 1 mm was inserted in the middle of the hypertrophic zone of the distal growth plate of ulna. In 33 of 37 animals, vascular invasion was successfully interposed by the membrane filter. During 8 days, the cartilage growth plate was enlarged, making the thickness 3-fold greater than that of the nonoperated control side. Histological examination demonstrated that the hypertrophic zone was exclusively elongated. At the terminal end of the growth plate, hypertrophic chondrocytes extruded from their territorial matrix into the open cavity on the surface of the membrane filter. The progenies of hypertrophic chondrocytes (PHCs) were PCNA positive and caspase-3 negative. In situ hybridization studies demonstrated that PHCs did not express cartilage matrix proteins anymore but expressed bone matrix proteins. Immunohistochemical studies also emonstrated that the new matrix produced by PHCs contained type I collagen, osteonectin, and osteocalcin. Based on these results, we concluded that hypertrophic chondrocytes switched into bone-forming cells after vascular invasion was interposed in the normal growth plate.
掲載誌名
PLOS ONE
ISSN
19326203
出版者
PLOS
9
8
開始ページ
e104638
発行日
2014-08-14
備考
内容要旨・審査要旨・論文本文の公開:
内容要旨・審査要旨 : LID201411041001.pdf
論文本文 : LID201411041002.pdf
本論文は, 著者Tetsuya Enishiの学位論文として提出され, 学位審査・授与の対象となっている。
権利情報
Copyright: © 2014 Enishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ( https://creativecommons.org/licenses/by/4.0/ )
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第2778号
学位記番号
甲医第1223号
学位授与年月日
2014-10-23
学位名
博士(医学)
学位授与機関
徳島大学
部局
病院
医学系