ID 113689
タイトル別表記
青色発光ダイオードはオプシン3を介し大腸癌細胞のオートファジーを誘導する
著者
良元, 俊昭 徳島大学大学院医科学教育部(医学専攻)
Feng, Rui Tokushima University
キーワード
autophagy
blue light emitting diodes (LED)
colorectal cancer
Opsin 3 (Opn3)
photoreceptor
資料タイプ
学位論文
抄録
Light emitting-diodes (LED) have various effects on living organisms and recent studies have shown the efficacy of visible light irradiation from LED for anticancer therapies. However, the mechanism of LED’s effects on cancer cells remains unclear. The aim of the present study was to investigate the effects of LED on colon cancer cell lines and the role of photoreceptor Opsin 3 (Opn3) on LED irradiation in vitro. Human colon cancer cells (HT-29 or HCT-116) were seeded onto laboratory dishes and irradiated with 465-nm LED at 30 mW/cm2 for 30 minutes. Cell Counting Kit-8 was used to measure cell viability, and apoptosis and caspase 3/8 expression were evaluated by AnnexinV/PI and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Autophagy and expression of LC-3 and beclin-1 were also evaluated by autophagy assays, RT-PCR and Western blotting. We further tested Opn3 knockdown by Opn3 siRNA and the Gi/o G-protein inhibitor NF023 in these assays. Viability of HT-29 and HCT-116 cells was lower in 465-nm LED-irradiated cultures than in control cultures. LC-3 and beclin-1 expressions were significantly higher in LED-irradiated cultures, and autophagosomes were detected in irradiated cells. The reductive effect of cancer cell viability following blue LED irradiation was reversed by Opn3 knockdown or NF023 treatment. Furthermore, increased LC-3 and beclin-1 expression that resulted from blue LED irradiation was suppressed by Opn3 knockdown or NF023 treatment. Blue LED irradiation suppressed the growth of colon cancer cells and Opn3 may play an important role as a photoreceptor.
掲載誌名
Annals of Gastroenterological Surgery
ISSN
24750328
出版者
The Japanese Society of Gastroenterological Surgery|Wiley
2
2
開始ページ
154
終了ページ
161
発行日
2018-01-11
備考
内容要旨・審査要旨・論文本文の公開
本論文は,著者Toshiaki Yoshimotoの学位論文として提出され,学位審査・授与の対象となっている。
著者の英語表記に誤植(誤)Takahiro Enomoto(正)Takahiro Emoto
権利情報
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ )
© 2018 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery
EDB ID
出版社版DOI
出版社版URL
フルテキストファイル
言語
eng
著者版フラグ
博士論文全文を含む
文科省報告番号
甲第3320号
学位記番号
甲医第1422号
学位授与年月日
2019-06-27
学位名
博士(医学)
学位授与機関
徳島大学
部局
医学系
病院
理工学系